![Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Azide-and-BSA-Free-Western-Blot-NBP2-80690-img0002.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate. Image from the standard format of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | 10D774 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ARHGDIB |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 0.02% gelatin. |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for D4-GDI/RhoGDI2 Antibody (NBP2-80690)Find related products by research area.
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D4-GDI (GDP dissociation inhibitor, RhoGD12) The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M... Read full blog post. |
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| Gene Symbol | ARHGDIB |
![Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80690-img0003.jpg "Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI. Image from the standard format of this antibody.")
![Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-10D774-Azide-and-BSA-Free-Western-Blot-NBP2-80690-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) - Azide and BSA Free [NBP2-80690] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.")
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