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D4-GDI/RhoGDI2 Antibody

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Western Blot: D4-GDI/RhoGDI2 Antibody [NBP2-33608] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA ...read more
Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody [NBP2-33608] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in hematopoietic cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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D4-GDI/RhoGDI2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PEPHVEEDDDDELDSKLNYKPPPQKSLKELQEMDKDDE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGDIB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
D4-GDI/RhoGDI2 Protein (NBP2-33608PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for D4-GDI/RhoGDI2 Antibody

  • ARHGDIB Rho GDP dissociation inhibitor (GDI) beta
  • D4
  • GDIA2
  • GDID4
  • LYGDI
  • Ly-GDI
  • RAP1GN1
  • RhoGDI2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on D4-GDI/RhoGDI2.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

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Bioinformatics

Gene Symbol ARHGDIB
Uniprot