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CD14 Antibody (M5E2) [PE]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77758PE
    • Availability
      Product Discontinued

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CD14 Antibody (M5E2) [PE] Summary

Immunogen
Human peripheral blood cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Simple Western
Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections.
Reviewed Applications
Read 1 Review rated 5
using
NB100-77758PE in the following applications:

Reactivity Notes

Expected to cross react with Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Cotton-topped Tamarin, Common Marmoset, Squirrel Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD14 Antibody (M5E2) [PE]

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NB100-77758PE) (0)

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Review for CD14 Antibody (NB100-77758PE) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-77758PE:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD14 NB100-77758PE
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5
reviewed by:
Verified Customer
Flow Human 11/23/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

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Video Protocols

ICC/IF Video Protocol

FAQs for CD14 Antibody (NB100-77758PE) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD14 Antibody (NB100-77758PE)

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Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Recent Reviews

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1
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Verified Customer
11/23/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD14