WRN Antibody

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner Syndrome, an autosomal recessive disorder characterized by premature aging.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Chemotaxis, Chromatin Immunoprecipitation (ChIP), ICC/IF, IF/IHC, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NB100-471	Applications	Species
Maresca C, Dello Stritto A, D'Angelo C et al. PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment Communications biology 2023-03-02 [PMID: 36864251] (Chromatin Immunoprecipitation (ChIP), Human)	Chromatin Immunoprecipitation (ChIP)	Human
Datta A, Biswas K, Sommers JA Et al. WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cells Nature communications 2021-11-12 [PMID: 34772932] (ICC/IF)	ICC/IF
Thangavel S, Berti M, Levikova M et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol 2015-03-02 [PMID: 25733713] (Human)		Human
Maddukuri L, Ketkar A, Eddy S et al. The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DnA polymerase kappa. Nucleic Acids Res. 2014-10-07 [PMID: 25294835] (ICC/IF, Human)	ICC/IF	Human
Betous R, Glick GG, Zhao R, Cortez D. Identification and Characterization of SMARCAL1 Protein Complexes. PLoS One 2013-05-09 [PMID: 23671665] (WB, Human)	WB	Human
Maddukuri L, Ketkar A, Eddy S et al. Enhancement of human DNA polymerase Eta activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein. J Biol Chem. 2012-12-07 [PMID: 23045531] (WB)	WB
Thangavel S, Mendoza-Maldonado R, Tissino E et al. Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol 2010-03-01 [PMID: 20065033] (Chemotaxis, Human)	Chemotaxis	Human
Rizzo A, Salvati E, Porru M et al. Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway. Nucleic Acids Res 2009-09-01 [PMID: 19596811]
Harrigan JA, Piotrowski J, Di Noto L et al. Metal-catalyzed Oxidation of the Werner Syndrome Protein Causes Loss of Catalytic Activities Impaired Protein-Protein Interactions. J Biol Chem, 2007;282(50):36403-11. 2007-12-14 [PMID: 17911100]
Das A, Boldogh I, Lee JW et al. The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1. J Biol Chem 2007-09-01 [PMID: 17611195]
Chen H, Wu X, Wang X et al. Downregulation of RECQL4 inhibits gastric cancer cell proliferation and induces cell cycle arrest at G0/G1 phase. Veterinary Microbiology. (WB, Human)	WB	Human
Hayashi K, Tasaka T, Kondo T et al. Successful Cord Blood Transplantation in a Werner Syndrome Patient with High-risk Myelodysplastic Syndrome Intern. Med. 2018-08-24 [PMID: 30146558] (IF/IHC, Human)	IF/IHC	Human
Nyunoya T, Monick MM, Klingelhutz AL et al. Cigarette Smoke Induces Cellular Senescence via Werner's Syndrome Protein Downregulation. Am J Respir Crit Care Med :200802-200320OC. 2008-01-01 [PMID: 19011155]
Show All 13 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Ingrid MONTARRAS	WB	Human	12/09/2019	View Summary Application Western Blot Sample Tested Colon cancer cell line Species Human Lot A2 Comments Comments Ab used at 1/1000_ON
Enlarge	4 reviewed by: Verified Customer	IF	Human	01/12/2015	View Summary Application Immunofluorescence Sample Tested Asynchronous HeLa cells Species Human Lot A2

Array NB100-471

• WRN Antibodies
• WRN Protein