![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0005.jpg "Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, HaSpecies Glossary |
| Applications | WB, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. |
| Specificity | Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | XRCC5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) This antibody has only been tested in Western blot. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Yongkang Yang |
WB | Human and Mouse | 10/10/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Ku80/XRCC5 Antibody (NB100-508)Find related products by research area.
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![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0004.jpg "Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.")
![Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunoprecipitation-NB100-508-img0003.jpg "Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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