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VAMP-2 Antibody

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Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed ...read more
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: VAMP-2 protein stained ...read more
Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: VAMP-2 protein stained by ...read more
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 ...read more
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 ...read more
Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VAMP-2 antibody ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VAMP-2 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP-2. The exact sequence is proprietary.
Predicted Species
Human (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAMP2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for VAMP-2 Antibody

  • FLJ11460
  • SYB2
  • SYB2vesicle-associated membrane protein 2
  • synaptobrevin 2
  • synaptobrevin-2
  • VAMP2
  • VAMP-2
  • VAMP-2vesicle associated membrane protein 2
  • vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VAMP-2 Antibody (NBP3-13179) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VAMP2