![Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Western-Blot-NBP2-75704-img0007.jpg "Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JG35-11 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human USP9x aa 1-118 / 2,554. (SwissProt: Q93008 Human; SwissProt: P70398 Mouse; SwissProt: D3ZC84 Rat) |
| Localization | Cell projection, Cytoplasm, Cytoskeleton. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | USP9X |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 290 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for USP9x Antibody (NBP2-75704)Find related products by research area.
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| Gene Symbol | USP9X |
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0004.jpg "Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0006.jpg "Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.")
![Flow Cytometry: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Flow-Cytometry-NBP2-75704-img0001.jpg "Flow Cytometry: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0002.jpg "Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0003.jpg "Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0005.jpg "Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")