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UPF2 Antibody

Images

 
Western Blot: UPF2 Antibody [NBP2-20813] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: UPF2 Antibody [NBP2-20813] - C. Effects of CC on the protein levels of NMD factors UPF1, UPF2, UPF3B, SMG1, SMG5, SMG6, and SMG7 in U2OS reporter cells. Image collected and cropped by CiteAb from the ...read more
Immunocytochemistry/ Immunofluorescence: UPF2 Antibody [NBP2-20813] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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UPF2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UPF2. The exact sequence is proprietary.
Localization
Cytoplasm; perinuclear region
Predicted Species
Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UPF2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
148 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-20813 in the following applications:

  • WB
    2 publications

Reactivity Notes

Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for UPF2 Antibody

  • DKFZP434D222
  • HUPF2
  • KIAA1408MGC138835
  • Nonsense mRNA reducing factor 2
  • regulator of nonsense transcripts 2
  • RENT2MGC138834
  • smg-3
  • UPF2 regulator of nonsense transcripts homolog (yeast)
  • Up-frameshift suppressor 2 homolog
  • yeast Upf2p homolog

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for UPF2 Antibody (NBP2-20813)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol UPF2
Uniprot