Genetic Strategies: Western Blot: UBTF Antibody [NBP1-82545] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: UBTF Antibody [NBP1-82545] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong positivity in neurons in the CA1 and granular cell layers in the hippocampus.
Western Blot: UBTF Antibody [NBP1-82545] - Analysis in mouse cerebral cortex tissue.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: UBTF Antibody [NBP1-82545] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse basal forebrain shows moderate nuclear positivity in neurons in the caudate putamen.
Immunohistochemistry: UBTF Antibody [NBP1-82545] - Staining of mouse brain shows strong nuclear positivity in neurons in the cerebral cortex.
This antibody was developed against Recombinant Protein corresponding to amino acids: WKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKK
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBTF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for UBTF Antibody
90-KDa Nucleolus Organizer Region Autoantigen
Autoantigen NOR-90
NOR-90
nucleolar transcription factor 1
UBF
UBF1
UBF-1
UBF2
UBFUBF-1
upstream binding transcription factor, RNA polymerase I
Upstream-binding factor 1
Background
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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