HMGB1/HMG-1 Antibody

HMGB1 (high mobility group protein B1) is a member of HMGB family and it has been extensively studied as a transcription/growth factor that is most ubiquitous and evolutionarily conserved protein in eukaryotic organisms. HMGB1 is localized in nucleus, however gets translocated to cytoplasm or may get secreted also. It is a DNA binding protein that preferentially binds with ssDNA and contains 2 HMG box DNA-binding domains with ability of chromatin association for bending DNA. HMGB1 implicates in in V(D)J recombination by acting as a cofactor of RAG complex that contains core components RAG1 and RAG2, as well as an associated component HMGB1 or HMGB2. HMGB1 acts by stimulating cleavage and RAG protein binding at 23bp spacer of conserved RSS (recombination signal sequences). Originally described as a nonhistone nuclear protein with transcriptional regulatory properties, HMGB1 (especially the cytoplasmic/secreted form) has also now been recognized as a late mediator in septic shock as well as a pro-inflammatory cytokine. It gets translocated to cytoplasm or secreted when cells get exposed to inflammatory mediators (LPS, TNF-alpha, IFN-gamma) or via passive process during cellular necrosis. HMGB1 has been implicated in the pathogenesis of various inflammatory and autoimmune diseases, such as SLE, RA and ankylosing spondylitis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 6 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, WB.

Showing Publications 1 - 10 of 32. Show All 32 Publications. Collapse Publications.

Publications using NB100-2322	Applications	Species
Abbaszadeh F, Jorjani M, Joghataei MT et al. Astaxanthin ameliorates spinal cord edema and astrocyte activation via suppression of HMGB1/TLR4/NF-?B signaling pathway in a rat model of spinal cord injury Naunyn-Schmiedeberg's archives of pharmacology 2023-05-05 [PMID: 37145127] (WB, Rat)	WB	Rat
Hao Y, Gu Z, Yu Z et al. Photodynamic Therapy in Combination with the Hepatitis B Core Virus-like Particles (HBc VLPs) to Prime Anticancer Immunity for Colorectal Cancer Treatment Cancers 2022-05-31 [PMID: 35681703] (FLOW, Mouse)	FLOW	Mouse
Lin T, Sheng L, Wan L et al. Suppressing HMGB1-TLR4-Mediated Neuroinflammation Alleviates Morphine Tolerance via Inhibiting AMPK-HO-1 Pathway in The Spinal Cord of Mice Research Square 2020-12-16 (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Yang W, Hong SA, Kim JM et al. The immunologic phenotype of thrombi is associated with future vascular events after cerebral infarction Journal of neurointerventional surgery 2023-05-17 [PMID: 37197936]
Liu J, Liu Y, Wang Y et al. HMGB1 is a mediator of cuproptosis-related sterile inflammation Frontiers in Cell and Developmental Biology 2022-09-21 [PMID: 36211458]
Ershova ES, Shmarina GV, Porokhovnik LN Et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls Genes (Basel) 2022-03-25 [PMID: 35328103] (FLOW, Human) Details: Citation using the FITC version of this antibody.	FLOW	Human
Kuwar R, Rolfe A, Di L et al. A Novel Inhibitor Targeting NLRP3 Inflammasome Reduces Neuropathology and Improves Cognitive Function in Alzheimer's Disease Transgenic Mice Journal of Alzheimer's Disease 2021-08-17 [PMID: 34219728] (WB)	WB
Baker BC, Heazell AEP, Sibley C et al. Hypoxia and oxidative stress induce sterile placental inflammation in vitro Scientific reports 2021-03-31 [PMID: 33790316] (IF/IHC, Human)	IF/IHC	Human
Ghaffari, S, Jang, E Et al. Endothelial HMGB1 Is a Critical Regulator of LDL Transcytosis via an SREBP2-SR-BI Axis. Arterioscler Thromb Vasc Biol 2021-01-01 [PMID: 33054399] (IF/IHC, Mouse)	IF/IHC	Mouse
Jella KK, Nasti TH, Li Z et al. Exosome-containing preparations from post-irradiated mouse melanoma cells delay melanoma growth in vivo by a natural killer cell-dependent mechanism Int. J. Radiat. Oncol. Biol. Phys. 2020-06-16 [PMID: 32561502]
Manzoor, S, Mariappan, N Et al. Cutaneous lewisite exposure causes acute lung injury. Ann N Y Acad Sci 2020-11-01 [PMID: 32329907] (IF/IHC, Mouse)	IF/IHC	Mouse
Evran S, Calis F, Akkaya E et al. The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model Brain Res. Bull. 2019-11-09 [PMID: 31715313] (IF/IHC, Rat)	IF/IHC	Rat
Domingo-Vidal M, Whitaker-Menezes D, Martos-Rus C et al. Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma Mol. Cancer Res. 2019-06-25 [PMID: 31239287] (ICC/IF, Rat)	ICC/IF	Rat
Li T, Liu B, Guan H et al. PGE2 increases inflammatory damage in Escherichia coli-infected bovine endometrial tissue in vitro via the EP4-PKA signaling pathway. Biol. Reprod. 2018-07-11 [PMID: 30010723]
Gao R, Ma J, Wen Z et al. Tumor cell-released autophagosomes (TRAP) enhance apoptosis and immunosuppressive functions of neutrophils Oncoimmunology. 2018-03-06 [PMID: 29872581] (WB, Mouse, Human) Details: Citation used the Alexa Fluor 488 format of this antibody.	WB	Mouse, Human
Kim SY, Son M, Lee SE et al. High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation. Front Immunol 2018-04-11 [PMID: 29696019] (WB, Mouse)	WB	Mouse
Liu X, Cao H, Li J et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. Cell Death Differ. 2017-04-01 [PMID: 28157209] (B/N)	B/N
Coleman LG, Zou J, Crews FT Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7 J Neuroinflammation Jan 25 2017 [PMID: 28118842] (FLOW, ELISA, Human) J Neuroinflammation. 2017-01-25 [PMID: 28118842] (ELISA, FLOW, Human) Details: Citation using the Alexa Fluor 488 form of this antibody.	ELISA, FLOW	Human
Zhou M, Wen Z, Cheng F et al. Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-kappa B signal pathway Oncoimmunology. 2016-05-12 [PMID: 27622036] (WB, Human)	WB	Human
Kim JS, Lee SG, Oh J et al. Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model Yonsei Med. J. 2016-09-01 [PMID: 27401639] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Feng Y, Yang S, Ma Y et al. Role of Toll-like receptors in diabetic renal lesions in a miniature pig model. Sci Adv 2015-06-01 [PMID: 26601192] (IF/IHC, Bovine)	IF/IHC	Bovine
Zahedi K, Barone S, Wang Y et al. Proximal tubule epithelial cell specific ablation of the spermidine/spermine n1-acetyltransferase gene reduces the severity of renal ischemia/reperfusion injury. PLoS OnE. 2014-11-13 [PMID: 25390069] (WB, Human)	WB	Human
Chi-Cheng Lu, Bo-Ru Huang, Pei-Jyuan Liao, Gow-Chin Yen. Ursolic acid triggers nonprogrammed death (necrosis) in human glioblastoma multiforme DBTRG-05MG cells through MPT pore opening and ATP decline. Mol Nutr Food Res. 2014-11-01 [PMID: 25131308]
Robinson AP, Caldis MW, Harp CT et al. High-mobility group box 1 protein (HMGB1) neutralization ameliorates experimental autoimmune encephalomyelitis. J Autoimmun 2013-01-01 [PMID: 23514872] (ICC/IF, Mouse)	ICC/IF	Mouse
Kang R, Tang D, Schapiro NE et al. The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics. Oncogene 2013-01-14 [PMID: 23318458] (WB, Mouse)	WB	Mouse
Montie HL, Pestell RG, Merry DE. SIRT1 Modulates Aggregation and Toxicity through Deacetylation of the Androgen Receptor in Cell Models of SBMA. J Neurosci 3031(48):17425-17436. 2011-11-01 [PMID: 22131404]
Tamura Yoshiaki, Chiba Yuko, Tanioka Toshihiro et al. NO donor induces Nec-1-inhibitable, but RIP1-independent, necrotic cell death in pancreatic B-cells. Febs Letters 2011-01-01 [PMID: 21888904] (WB, Mouse)	WB	Mouse
Tang D, Kang R, Livesey KM et al. High-mobility group box 1 is essential for mitochondrial quality control Cell Metab 2011-06-01 [PMID: 21641551] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Fazio EN, Dimattia GE, Chadi S et al. Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice BMC Cell Biol 2011-05-05 [PMID: 21545732] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Jiang Y, Scott KL, Kwak SJ et al. Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling. Oncogene 2011-07-21 [PMID: 21399659]
Barlan AU, Griffin TM, McGuire KA, Wiethoff CM. Adenovirus membrane penetration activates the NLRP3 inflammasome. J Virol. 2010-10-27 [PMID: 20980503] (WB, Human)	WB	Human
Show All 32 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: seulgee lee	IHC-P	Rabbit	04/24/2018	View Summary Application Immunohistochemistry-Paraffin Sample Tested veesel Species Rabbit Lot D-1
Enlarge	5 reviewed by: Verified Customer	WB	Rat	03/28/2018	View Summary Application Western Blot Sample Tested Brain homogenate Species Rat Lot Q-1 Comments Comments Blocked in 5% milk + 1% BSA in TBS for 2 h, incubated overnight in 1:500 HMGB1 antibody at 4 degrees C and then 2 h at room temperature with HRP-conjugated secondary.
Enlarge	4 reviewed by: Liyong Zhang	WB	Human	11/25/2017	View Summary Application Western Blot Sample Tested mouse hepatocytes and HepG2 cells Species Human Lot F-1
Enlarge	4 reviewed by: Verified Customer	Simple Western	Mouse	12/30/2015	View Summary Application Simple Western Sample Tested mouse serum Species Mouse
Enlarge	5 reviewed by: Liyong Zhang	WB	Mouse	06/26/2014	View Summary Application Western Blot Species Mouse

Array NB100-2322

• HMGB1/HMG-1 Antibodies
• HMGB1/HMG-1 Antibody Pair
• HMGB1/HMG-1 ELISA Kits
• HMGB1/HMG-1 Lysate
• HMGB1/HMG-1 Proteins
• HMGB1/HMG-1 Proteins and Enzyme