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SSRP1 Antibody

 
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Western Blot: SSRP1 Antibody [NBP1-84754] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High
Western Blot: SSRP1 Antibody [NBP1-84754] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High
Immunohistochemistry-Paraffin: SSRP1 Antibody [NBP1-84754] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Simple Western: SSRP1 Antibody [NBP1-84754] - Simple Western lane view shows a specific band for SSRP1 in 0.2 mg/ml of Ntera-2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: SSRP1 Antibody [NBP1-84754] - Electropherogram image of the corresponding Simple Western lane view. SSRP1 antibody was used at 1:25 dilution on Ntera-2 lysate(s) respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SSRP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVE
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SSRP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:25
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Control Peptide
SSRP1 Protein (NBP1-84754PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SSRP1 Antibody

  • Chromatin-specific transcription elongation factor 80 kDa subunit
  • cisplatin-DNA SSRP
  • facilitates chromatin remodeling 80 kDa subunit
  • Facilitates chromatin transcription complex 80 kDa subunit
  • Facilitates chromatin transcription complex subunit SSRP1
  • FACT complex subunit SSRP1
  • FACT
  • FACT80FACT 80 kDa subunit
  • FACTp80
  • high mobility group box
  • hSSRP1
  • Recombination signal sequence recognition protein 1
  • structure specific recognition protein 1
  • Structure-specific recognition protein 1
  • T160

Background

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D7 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SSRP1