UBAP1 Antibody

Images

 
Western Blot: UBAP1 Antibody [NBP1-80651] - Analysis of UBAP1 in Panc-1 and AsPC-1 whole cell lysates using anti-UBAP1 antibody. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: UBAP1 Antibody [NBP1-80651] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
Immunohistochemistry-Paraffin: UBAP1 Antibody [NBP1-80651] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

UBAP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FHGTFSYLDDVPFKTGDKFKTPAKVGLPIGFSLPDCLQVVREVQYDFSLEKKTIEWAEEIKKIEEAEREAECKIAEAEAKVNSKSGPEGDSKMSFSKTHS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot Validated from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, PFA/Triton X-100.
Control Peptide
UBAP1 Protein (NBP1-80651PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-80651 in the following applications:

Publications
Read Publications using NBP1-80651.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%). Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UBAP1 Antibody

  • MGC119669
  • MGC8710
  • NAG20
  • Nasopharyngeal carcinoma-associated gene 20 protein
  • UAP
  • UBAP
  • UBAP-1
  • ubiquitin associated protein 1
  • ubiquitin associated protein
  • ubiquitin-associated protein 1

Background

UBAP1 is a member of the UBA domain family, whose members include proteins having connections to ubiquitin and the ubiquitination pathway. The ubiquitin associated domain is thought to be a non-covalent ubiquitin binding domain consisting of a compact three helix bundle. This particular protein originates from a gene locus in a refined region on chromosome 9 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Taking into account its cytogenetic location, this UBA domain family member is being studies as a putative target for mutation in nasopharyngeal carcinomas. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBAP1 Antibody (NBP1-80651)(3)

Review for UBAP1 Antibody (NBP1-80651) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-80651:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot UBAP1 NBP1-80651
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4
reviewed by:
Verified Customer
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedPanc-1 and AsPC-1 whole cell lysates
SpeciesHuman
LotR09662

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UBAP1 Antibody (NBP1-80651) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional UBAP1 Products

Blogs on UBAP1.

Chinese Cancer Study Reveals Three New Genes for Nasopharyngeal Carcinomas
A large percentage of the products in our antibody catalog are used for cancer research. Some oncogenes are expressed in several types of tumor, while others are quite specific. For example, there are several products on our antibody database which ta...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

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Verified Customer
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBAP1
Entrez