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Acetyl CoA synthetase Antibody

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Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human tonsil shows no positivity in lymphocytes as expected.
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human stomach shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human Fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Acetyl CoA synthetase Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PGETTQITYHQLLVQVCQFSNVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCSLLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQ
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Acetyl CoA synthetase Protein (NBP1-90276PEP)
Publications
Read Publications using
NBP1-90276 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20232292), Mouse reactivity reported in the literature (PMID: 25584894)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Acetyl CoA synthetase Antibody

  • ACAS2
  • AceCS
  • acetate thiokinase
  • acetate-CoA ligase
  • Acetate--CoA ligase
  • Acetyl-CoA synthetase
  • acetyl-Coenzyme A synthetase 2 (ADP forming)
  • acetyl-coenzyme A synthetase, cytoplasmic
  • ACSA
  • ACSACECS
  • Acyl-activating enzyme
  • acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
  • cytoplasmic acetyl-coenzyme A synthetase
  • dJ1161H23.1
  • DKFZp762G026
  • EC 6.2.1

Background

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Acetyl CoA synthetase Antibody (NBP1-90276)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACSS2
Entrez
Uniprot