Reactivity | HuSpecies Glossary |
Applications | IHC |
Clone | T311 + OCA1/812 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | IHC-Prediluted |
Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
Immunogen | Recombinant tyrosinase protein (Uniprot: P14679 ) |
Localization | Cytoplasmic |
Marker | Melanoma Marker |
Specificity | Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This monoclonal antibody shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this monoclonal antibody shows tyrosinase in melanotic as well as amelanotic variants. This monoclonal antibody is a useful marker for melanocytes and melanomas. |
Isotype | IgG2a Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | TYR |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Hu-chromosome location: 11q14.3 Molecular weight of antigen: 70-80kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM Citrate buffer, pH6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Tyrosinase Antibody (NBP2-48119)Find related products by research area.
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Gene Symbol | TYR |