Home » Tumstatin/COL4A3 » COL4A3 Antibodies » Tumstatin/COL4A3 Antibody (762102)
Novus Biologicals products are now on bio-techne.com

Tumstatin/COL4A3 Antibody (762102)

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tumstatin Monoclonal Antibody (Catalog # MAB7546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tumstatin at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tumstatin Monoclonal Antibody (Catalog # MAB7546) followed by HRP-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
762102
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7546-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Tumstatin/COL4A3 Antibody (762102) Summary

Immunogen
E. coli-derived recombinant human Tumstatin
Specificity
Detects human Tumstatin in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human Collagen IV alpha 1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COL4A3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tumstatin/COL4A3 Antibody (762102)

  • COL4A3
  • collagen alpha-3(IV) chain
  • collagen IV, alpha-3 polypeptide
  • collagen, type IV, alpha 3 (Goodpasture antigen)
  • Goodpasture antigen
  • Tumstatin

Background

Tumstatin is a naturally occurring, anti-angiogenic, anti-tumor peptide of 28 kDa that constitutes the NC1 (non-collagenous) domain of the Collagen IV a3 chain [amino acids (aa) 1427-1670 of Coll IV a3;]. It is liberated from basement membranes by MMP-9, and interacts with Integrin aVb3 on endothelial cells, causing cell cycle arrest, or apoptosis of cells that are proliferating. Human Tumstatin is also active on mouse and bovine endothelial cells. Human Tumstatin shares 91% and 89% aa sequence identity with mouse and rat Tumstatin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Collagen IV alpha4
Antibody
NBP3-43374
Collagen IV alpha4 Antibody
Species: Hu
Applications: ICC/IF, IHC, IP, WB
Collagen IV alpha5
Antibody
NBP3-41417
Western Blot Collagen IV alpha5 Antibody
Western Blot Collagen IV alpha5 Antibody
Western Blot Collagen IV alpha5 Antibody
Species: Hu, Mu, Po, Rt
Applications: WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
Endostatin [HRP]
DNST0
N/A Endostatin [HRP]
N/A Endostatin [HRP]
(22 Publications)
Species: Hu
Applications: ELISA
Thrombospondin-1
[HRP]
DTSP10
N/A Thrombospondin-1 [HRP]
(42 Publications)
Species: Hu
Applications: ELISA
COL4A2 Antibody
NBP3-41311
Immunohistochemistry-Paraffin COL4A2 Antibody
Western Blot COL4A2 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, WB
Collagen IV
Antibody
NB120-6586
Immunohistochemistry-Paraffin Collagen IV Antibody
Immunohistochemistry-Paraffin Collagen IV Antibody
Immunohistochemistry-Paraffin Collagen IV Antibody
(7 Reviews)
(43 Publications)
Species: Bv, Fe, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, KO, SR Microscopy, WB
Recombinant Human
COL4A6 GST (N-Term) P ...
H00001288-P02
Other Recombinant Human COL4A6 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, WB
CTGF/CCN2 Antibody
[Unconjugated] - C-te ...
AF660
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus
Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus
(7 Publications)
Species: Hu
Applications: IHC
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(220 Publications)
Species: Hu
Applications: ELISA
Integrin alpha V
beta 3 [Unconjugated]
3050-AV
Integrin alpha V beta 3 [Unconjugated]
(25 Publications)
Species: Hu
Applications: Bind
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody
Western Blot TOR/mTOR [p Ser2448] Antibody
Data TOR/mTOR [p Ser2448] Antibody
(2 Reviews)
(9 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
FAK Antibody
[Unconjugated]
AF4467
Immunohistochemistry FAK Antibody [Unconjugated]
Knockout Validated FAK Antibody [Unconjugated]
Western Blot FAK Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Angiopoietin-2
[HRP]
DANG20
N/A Angiopoietin-2 [HRP]
N/A Angiopoietin-2 [HRP]
(103 Publications)
Species: Hu
Applications: ELISA

Publications for Tumstatin/COL4A3 Antibody (MAB7546) (0)

There are no publications for Tumstatin/COL4A3 Antibody (MAB7546).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Tumstatin/COL4A3 Antibody (MAB7546) (0)

There are no reviews for Tumstatin/COL4A3 Antibody (MAB7546). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Tumstatin/COL4A3 Antibody (MAB7546) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Tumstatin/COL4A3 Products

Blogs on Tumstatin/COL4A3

There are no specific blogs for Tumstatin/COL4A3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Tumstatin/COL4A3 Antibody (762102) and receive a gift card or discount.


Bioinformatics

Gene Symbol COL4A3