![Western Blot: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of extracts of various cell lines, using Tropomyosin-1 Rabbit mAb (NBP3-16689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Western-Blot-NBP3-16689-img0005.jpg "Western Blot: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of extracts of various cell lines, using Tropomyosin-1 Rabbit mAb (NBP3-16689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | 5Q2H6 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 185-284 of human Tropomyosin-1 (P09493). LSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TPM1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry MYH7 Antibody (940906) [Unconjugated]](https://images.novusbio.com/images2/MYH7_MAB9096_Immunohistochemistry_19508.jpg)
![Western Blot MYH7 Antibody (940906) [Unconjugated]](https://images.novusbio.com/images2/MYH7_MAB9096_Western_Blot_19525.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Tropomyosin-1 Antibody (NBP3-16689)Find related products by research area.
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| Gene Symbol | TPM1 |
![Immunohistochemistry: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of mouse heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Immunohistochemistry-NBP3-16689-img0004.jpg "Immunohistochemistry: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of mouse heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Rat heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Immunohistochemistry-Paraffin-NBP3-16689-img0001.jpg "Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Rat heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Mouse heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Immunohistochemistry-Paraffin-NBP3-16689-img0002.jpg "Immunohistochemistry-Paraffin: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Mouse heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of rat heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Immunohistochemistry-NBP3-16689-img0003.jpg "Immunohistochemistry: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of rat heart using Tropomyosin-1 Rabbit mAb (NBP3-16689) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunoprecipitation: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of 600ug extracts of Rat heart cells using 3ug Tropomyosin-1 antibody (NBP3-16689). Western blot was performed from the immunoprecipitate using Tropomyosin-1 antibody (NBP3-16689) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Tropomyosin-1-Antibody-5Q2H6-Immunoprecipitation-NBP3-16689-img0006.jpg "Immunoprecipitation: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Analysis of 600ug extracts of Rat heart cells using 3ug Tropomyosin-1 antibody (NBP3-16689). Western blot was performed from the immunoprecipitate using Tropomyosin-1 antibody (NBP3-16689) at a dilition of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Immunofluorescence analysis of paraffin-embedded mouse heart using Tropomyosin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16689_rabbit-tropomyosin-1-mab-5q2h6-5220251215419.jpg "Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (5Q2H6) [NBP3-16689] - Immunofluorescence analysis of paraffin-embedded mouse heart using Tropomyosin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (5Q2H6) [Tropomyosin-1] - Immunofluorescence analysis of paraffin-embedded mouse heart using Tropomyosin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16689_rabbit-tropomyosin-1-mab-5q2h6-52202517315528.jpg "Immunocytochemistry/ Immunofluorescence: Tropomyosin-1 Antibody (5Q2H6) [Tropomyosin-1] - Immunofluorescence analysis of paraffin-embedded mouse heart using Tropomyosin-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")