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TRIM21/SSA1 Antibody - Azide and BSA Free

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Genetic Strategies: Western Blot: TRIM21/SSA1 Antibody [NBP3-03809] - Analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21/SSA1 antibody at 1:1000 dilution. Secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

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TRIM21/SSA1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21/SSA1 (NP_003132.2). SQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM21
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Knockout Validated
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for TRIM21/SSA1 Antibody - Azide and BSA Free

  • 52 kDa ribonucleoprotein autoantigen Ro/SS-A
  • 52 kDa Ro/SSA
  • E3 ubiquitin-protein ligase TRIM21
  • EC 6.3.2.-
  • rhTRIM21
  • RNF81
  • RNF81RING finger protein 81
  • ro(SS-A)
  • Ro52
  • RO52SS-A
  • Sicca syndrome antigen A
  • Sjoegren syndrome type A antigen
  • Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
  • SSA1
  • SSA1SSA
  • TRIM21
  • tripartite motif containing 21,52 kDa Ro protein
  • tripartite motif-containing 21
  • Tripartite motif-containing protein 21

Background

SSA1 encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM21