TRF-2 Antibody (689524) Summary
Immunogen |
E. coli-derived recombinant human TRF2 Gln78-Thr238 (predicted) Accession # Q15554 |
Specificity |
Detects human TRF2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human TRF1 is observed. |
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
TERF2 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Sterile PBS to a final concentration of 0.5 mg/mL. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TRF-2 Antibody (689524)
Background
TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13-30), a dimerization domain (aa 46-112), an NLS (aa 329-333), and a DNA binding HTH myb-type domain (aa 442-499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239-500. Over aa 78-238, human TRF2 shares 96% aa identity with mouse TRF2.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for TRF-2 Antibody (MAB5635). (Showing 1 - 5 of 5 FAQ).
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Are the TRF-2 antibodies validated in Simple Western?
- Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
-
Does TRF-2 antibodies comes in lyophilized form?
- we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
-
What the theoretical molecular weight for TRF-2 antibodies?
- The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
-
Does this TRF-2 antibody come in lyophilized form?
- Yes, we carry this TRF-2 antibodies in lyophilized format.
-
What is the immunogen sequence of this TRF-2 antibody?
- A sequence from Gln78 - Thr238 (predicted).