Home » TRAILR3/TNFRSF10C » TRAILR3/TNFRSF10C Antibodies » TRAILR3/TNFRSF10C Antibody [Unconjugated]
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TRAILR3/TNFRSF10C Antibody [Unconjugated]

 (12 Publications)
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Western blot shows lysates of L-929 mouse fibroblast cell line, THP-1 human acute monocytic leukemia cell line, and WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human TRAIL R3/TNFRSF10C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TRAIL R3/TNFRSF10C at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of L-929 mouse fibroblast cell line, THP-1 human acute monocytic leukemia cell line, and WS-1 human fetal skin fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human ...read more
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (5 µg/mL, Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF630
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF630-SP
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TRAILR3/TNFRSF10C Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
Ala26-Ala221
Accession # O14798
Specificity
Detects human TRAIL R3/TNFRSF10C in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, and rhTRAIL R2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF10C
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.015-0.045 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF630 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR3/TNFRSF10C Antibody [Unconjugated]

  • Antagonist decoy receptor for TRAIL/Apo-2L
  • CD263 antigen
  • CD263
  • cytotoxic TRAIL receptor-3
  • DcR1
  • DCR1MGC149502
  • Decoy receptor 1
  • Decoy TRAIL receptor without death domain
  • LITMGC149501
  • Lymphocyte inhibitor of TRAIL
  • TNFRSF10C
  • TRAIL R3
  • TRAIL receptor 3
  • TRAIL receptor without an intracellular domain
  • TRAILR3
  • TRAIL-R3
  • TRAILR3TNF-related apoptosis-inducing ligand receptor 3
  • TRIDDCR1-TNFR
  • tumor necrosis factor receptor superfamily member 10C
  • tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

Background

Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. In the new TNF superfamily nomenclature, TRAIL R3 is referred to as TNFRSF10C. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

  1. Sheridan, J.P. et al. (1997) Science 277:818.
  2. Golstein, P. (1997) Curr. Biol. 7:R750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAILR3/TNFRSF10C Antibody (AF630)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: Cell Culture, Flow Cytometry, ICC, IHC-P, Neutralization, Western Blot.


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Cell Culture
(1)
Flow Cytometry
(4)
ICC
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(2)
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Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF630 Applications Species
Johannes Höfle, Timo Trenkner, Nadja Kleist, Vera Schwane, Sarah Vollmers, Bryan Barcelona et al. Engagement of TRAIL triggers degranulation and IFN gamma production in human natural killer cells EMBO reports 8/3/2022 [PMID: 35758160]
M Kopczynski, M Statkiewic, M Cybulska, U Kuklinska, K Unrug-Biel, Z Sandowska-, A Grochowska, M Gajewska, M Kulecka, J Ostrowski, M Mikula Cytotoxic Efficacy and Resistance Mechanism of a TRAIL and VEGFA-Peptide Fusion Protein in Colorectal Cancer Models International Journal of Molecular Sciences, 2021-03-19;22(6):. 2021-03-19 [PMID: 33808900] (ICC, Human) ICC Human
?? ?rtykov, DA Belov, VO Shipunova, DB Trushina, SM Deyev, DA Dolgikh, MP Kirpichnik, ME Gasparian Chemotherapeutic Agents Sensitize Resistant Cancer Cells to the DR5-Specific Variant DR5-B more Efficiently than to TRAIL by Modulating the Surface Expression of Death and Decoy Receptors Cancers (Basel), 2020-04-30;12(5):. 2020-04-30 [PMID: 32365976] (Cell Culture, Human) Cell Culture Human
M van Dijk, A Halpin-McCormick, T Sessler, A Samali, E Szegezdi Resistance to TRAIL in non-transformed cells is due to multiple redundant pathways Cell Death & Disease 7/4/2013 [PMID: 23828565]
Wu CH, Kao CH, Safa AR TRAIL recombinant adenovirus triggers robust apoptosis in multidrug-resistant HL-60/Vinc cells preferentially through death receptor DR5. Hum. Gene Ther., 2008-07-01;19(7):731-43. 2008-07-01 [PMID: 18476767] (Neutralization, Human) Neutralization Human
Zopf S, Neureiter D, Bouralexis S, Abt T, Glaser KB, Okamoto K, Ganslmayer M, Hahn EG, Herold C, Ocker M Differential response of p53 and p21 on HDAC inhibitor-mediated apoptosis in HCT116 colon cancer cells in vitro and in vivo. Int. J. Oncol., 2007-12-01;31(6):1391-402. 2007-12-01 [PMID: 17982666] (Western Blot, Human) Western Blot Human
Park SJ, Wu CH, Choi MR, Najafi F, Emami A, Safa AR P-glycoprotein enhances TRAIL-triggered apoptosis in multidrug resistant cancer cells by interacting with the death receptor DR5. Biochem. Pharmacol., 2006-05-04;72(3):293-307. 2006-05-04 [PMID: 16753135] (Flow Cytometry, Human) Flow Cytometry Human
Lane D, Cote M, Grondin R, Couture MC, Piche A Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3. Mol. Cancer Ther., 2006-03-01;5(3):509-21. 2006-03-01 [PMID: 16546965] (Western Blot, Human) Western Blot Human
Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis. Biochem. Biophys. Res. Commun., 2005-07-22;333(1):42-50. 2005-07-22 [PMID: 15936719] (IHC-P, Human) IHC-P Human
Zauli G, Pandolfi A, Gonelli A, Di Pietro R, Guarnieri S, Ciabattoni G, Rana R, Vitale M, Secchiero P Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sequentially upregulates nitric oxide and prostanoid production in primary human endothelial cells. Circ. Res., 2003-03-20;92(7):732-40. 2003-03-20 [PMID: 12649264] (Flow Cytometry, Human) Flow Cytometry Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol TNFRSF10C
Uniprot