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TPX2 Antibody (18D5-1)

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Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.
Western Blot: TPX2 Antibody (18D5-1) [NB100-74556] - Analysis of TPX2.
Immunohistochemistry-Paraffin: TPX2 Antibody (18D5-1) [NB100-74556] - Biopsies of deparaffinized Human testis tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
18D5-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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TPX2 Antibody (18D5-1) Summary

Immunogen
Recombinant human TPX2
Localization
Nuclear. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus.
Specificity
TPX2 (18D5-1)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPX2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 4 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 2 ug/mL
Control
TPX2 Overexpression Lysate
Publications
Read Publications using
NB100-74556 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TPX2 Antibody (18D5-1)

  • C20orf1HCTP4
  • C20orf2
  • chromosome 20 open reading frame 1
  • Differentially expressed in cancerous and non-cancerous lung cells 2
  • differentially expressed in lung cells
  • DIL2
  • DIL-2FLS353
  • HCA519
  • Hepatocellular carcinoma-associated antigen 519
  • P100
  • p100GD:C20orf1
  • preferentially expressed in colorectal cancer
  • Protein fls353
  • REPP86
  • restricted expression proliferation associated protein 100
  • Restricted expression proliferation-associated protein 100
  • targeting protein for Xklp2
  • TPX2, microtubule-associated protein homolog
  • TPX2, microtubule-associated, homolog (Xenopus laevis)

Background

TPX2 is essential for spindle pole and mitotic spindle formulation, playing a role in the S and G2 phases of mitosis. It is also critical for the function of protein kinase Aurora A. TPX2 autophosphorylates Aurora A, targeting it to the spindle apparatus and allowing it to function in eukaryotic meiotic and mitotic cell cycles. TPX2 has also been found to be highly expressed in cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TPX2 Antibody (18D5-1)
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Publications for TPX2 Antibody (NB100-74556)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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(1)
WB
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPX2 Antibody (NB100-74556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

TPX2 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPX2
Entrez