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Human TOR/mTOR ELISA Kit (Chemiluminescence)

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ELISA: Human TOR/mTOR ELISA Kit (Chemiluminescence) [NBP2-75349] - Samples were spiked with high concentrations of Human TOR/mTOR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human TOR/mTOR ELISA Kit (Chemiluminescence) [NBP2-75349] - Samples were spiked with high concentrations of Human TOR/mTOR and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human TOR/mTOR ELISA Kit (Chemiluminescence) [NBP2-75349] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

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Human TOR/mTOR ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human mTOR in samples. No significant cross-reactivity or interference between Human mTOR and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 10.73%
Intra-Assay
CV% < 8.9%
Spike Recovery
92-112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
MTOR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human TOR/mTOR ELISA Kit (Chemiluminescence)

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  Human TOR/mTOR ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol MTOR