I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

TNF-alpha Antibody [Biotin] (BAF210): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ELISA(Det), ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Biotin
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Standard Formulation AF-210-NA

Carrier Free AF-210-NA

Carrier Free AF-210-SP

Conjugate	Catalog #	Availability	Size	Price
Unconjugated	AF-210-NA

Immunogen	E. coli-derived recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA) Val77-Leu233 Accession # P01375
Specificity	Detects human TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) TNF‑ beta , recombinant mouse (rm) TNF-alpha , recombinant rat TNF‑ alpha , recombinant porcine TNF-alpha , rhsTNF RII, rmsTNF RI, and rmsTNF RII is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TNF
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

TNF-alpha Antibody [Biotin]

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Alternate Names for TNF-alpha Antibody [Biotin]

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Publications for TNF-alpha Antibody (BAF210)(38)

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FAQs for TNF-alpha Antibody (BAF210). (Showing 1 - 4 of 4 FAQs).

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using BAF210	Applications	Species
Doyeon Koo, Zhiyuan Mao, Robert Dimatteo, Natalie Tsubamoto, Miyako Noguchi, Jami McLaughlin et al. Defining T cell receptor repertoires using nanovial-based affinity and functional screening bioRxiv 6/15/2023 [PMID: 36711524]
S Banjonjit, M Taweechoti, S Rungsiyano Effect of probiotic Lactobacillus paracasei on tumor necrosis factor-alpha level in gingival crevicular fluid of patients undergoing impacted third molar removal Journal of oral science, 2022-04-08;0(0):. 2022-04-08 [PMID: 35400713] (ELISA Detection, Human)	ELISA Detection	Human
Darcy R Denner, Maria LD Udan-Johns, Michael R Nichols Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes World Journal of Biological Chemistry 1/27/2021 [PMID: 33552397]
DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF Nature Communications, 2021-01-25;12(1):583. 2021-01-25 [PMID: 33495445] (ELISA (detection), Human)	ELISA (detection)	Human
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
MF Abas?yan?k, K Wolfe, H Van Phan, J Lin, B Laxman, SR White, PA Verhoef, GM Mutlu, B Patel, S Tay Ultrasensitive digital quantification of cytokines and bacteria predicts septic shock outcomes Nat Commun, 2020-05-25;11(1):2607. 5/25/2020 [PMID: 32451375]
A Stumpf, T Brandstett, J Hübner, J Rühe Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum PLoS ONE, 2019-12-02;14(12):e0225525. 2019-12-02 [PMID: 31790441] (ELISA Detection, Human)	ELISA Detection	Human
Rikard Pehrson, Tove Hegelund-Myrbäck, Danen Cunoosamy, Sara Asimus, Paul Jansson, Naimish Patel et al. AZD7624, an Inhaled p38 Inhibitor, Demonstrates Local Lung Inhibition of LPS-Induced TNF alpha with Minimal Systemic Exposure Journal of Pharmacology and Experimental Therapeutics 6/1/2018 [PMID: 29549158]
E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017-04-14;12(4):e0175712. 2017-04-14 [PMID: 28410380] (ELISA Development (Detection), Human)	ELISA Development (Detection)	Human
A Deora, S Hegde, J Lee, CH Choi, Q Chang, C Lee, L Eaton, H Tang, D Wang, D Lee, M Michalak, M Tomlinson, Q Tao, N Gaur, B Harvey, S McLoughlin, B Labkovsky, T Ghayur Transmembrane TNF-dependent uptake of anti-TNF antibodies MAbs, 2017-03-21;0(0):0. 2017-03-21 [PMID: 28323513] (Western Blot, Human)	Western Blot	Human
SA Castro, R Collighan, PA Lambert, IH Dias, P Chauhan, CE Bland, I Milic, MR Milward, PR Cooper, A Devitt Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils Cell Death Dis, 2017-03-02;8(3):e2644. 2017-03-02 [PMID: 28252646] (Human)		Human
Matt P, Lindqvist U, Kleinau S Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. PLoS ONE, 2015-09-25;10(9):e0137474. 2015-09-25 [PMID: 26406605] (ELISA Development (Detection), Human)	ELISA Development (Detection)	Human
Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips BMC Biotechnology 12/1/2015 [PMID: 26268325]
Elizabeth England, Philip Newton, Frances Neal, Lisa Kitching, Caroline Colley, Christine J. Rossant Application of the Mirrorball High-Sensitivity Cytometer to Multiplexed Assays for Antibody Drug Discovery J Biomol Screen 4/1/2015 [PMID: 25381256]
Eva Melin, Azita Sohrabian, Johan Rönnelid, Kristian Borg Normal serum levels of immune complexes in postpolio patients Results in Immunology 6/18/2014 [PMID: 25009767]
Aurelie Bornot, Carolyn Blackett, Ola Engkvist, Clare Murray, Claus Bendtsen The Role of Historical Bioactivity Data in the Deconvolution of Phenotypic Screens J Biomol Screen 6/1/2014 [PMID: 24441646]
Kaori Abe, Yoshiko Hashimoto, Shouki Yatsushiro, Shohei Yamamura, Mika Bando, Yuka Hiroshima et al. Simultaneous Immunoassay Analysis of Plasma IL-6 and TNF-alpha on a Microchip PLoS ONE 1/9/2013 [PMID: 23326472]
Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011-07-09;54(10):2713-23. 2011-07-09 [PMID: 21744073] (ELISA Development, Human)	ELISA Development	Human
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF Protection from RNA and DNA viruses by IL-32. J. Immunol., 2011-02-23;186(7):4110-8. 2011-02-23 [PMID: 21346229] (Electrochemiluminescent Assay, Human)	Electrochemiluminescent Assay	Human
David C. Appleyard, Stephen C. Chapin, Patrick S. Doyle Multiplexed Protein Quantification with Barcoded Hydrogel Microparticles Analytical Chemistry 1/1/2011 [PMID: 21142122]
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Human)	ELISA Development	Human
Caroline Karlsson, Siv Ahrné, Göran Molin, Anna Berggren, Ingrid Palmquist, Gunilla Nordin Fredrikson et al. Probiotic therapy to men with incipient arteriosclerosis initiates increased bacterial diversity in colon: A randomized controlled trial Atherosclerosis 1/1/2010 [PMID: 19608185]
Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)	Antibody Array Development	Human
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)	ELISA Microarray Development	Human
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)	Luminex Development	Human
O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects. Clin. Exp. Allergy, 2008-02-25;38(4):594-601. 2008-02-25 [PMID: 18307523] (ELISA Development, Human)	ELISA Development	Human
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol., 2008-02-15;180(4):2553-61. 2008-02-15 [PMID: 18250466] (ELISA Development, Human)	ELISA Development	Human
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-11-01;53(11):2010-2. 2007-11-01 [PMID: 18030696] (ELISA Development, Human)	ELISA Development	Human
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-09-21;53(11):1990-5. 2007-09-21 [PMID: 17890441] (ELISA Development, Human)	ELISA Development	Human
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)	Luminex Development	Human
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)	Luminex Development	Human
Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S Sepsis and pathophysiology of anthrax in a nonhuman primate model. Am. J. Pathol., 2006-08-01;169(2):433-44. 2006-08-01 [PMID: 16877346] (ELISA Development, Primate - Papio cynocephalus (Yellow Baboon))	ELISA Development	Primate - Papio cynocephalus (Yellow Baboon)
Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB Selective regulation of IL-10 signaling and function by zymosan. J. Immunol., 2006-04-15;176(8):4785-92. 2006-04-15 [PMID: 16585572] (ELISA Development, Human)	ELISA Development	Human
Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Increased ENA-78 in the follicular fluid of patients with endometriosis. Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42. 2006-01-01 [PMID: 16553183] (ELISA Development, Human)	ELISA Development	Human
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)	ELISA Development	Human
Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J. Immunol., 2004-05-15;172(10):6476-81. 2004-05-15 [PMID: 15128840] (ELISA Development, Human)	ELISA Development	Human
Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (Array Development, Human)	Array Development	Human
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB Reprogramming of IL-10 activity and signaling by IFN-gamma. J. Immunol., 2003-11-15;171(10):5034-41. 2003-11-15 [PMID: 14607900] (ELISA Development, Human)	ELISA Development	Human
Show All 38 Publications. Collapse Publications.

Gene Symbol	TNF
Uniprot	P01375()