I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

TNF-alpha Antibody [Unconjugated] (AF-210-NA): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, B/N, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF210

Immunogen	E. coli-derived recombinant human TNF-alpha Val77-Leu233 Accession # P01375
Specificity	Detects human TNF‑ alpha in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	TNF
Purity Statement	Antigen Affinity-purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

TNF-alpha Antibody [Unconjugated]

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TNF-alpha Antibody [Unconjugated] Summary

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Publications for TNF-alpha Antibody (AF-210-NA)(41)

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FAQs for TNF-alpha Antibody (AF-210-NA). (Showing 1 - 4 of 4 FAQs).

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Bioinformatics

Publications using AF-210-NA	Applications	Species
Unnikrishnan M. Chandrasekharan, Raminderjit Kaur, Jennifer E. Harvey, Chad Braley, Vandana Rai, MacKenzie Lee et al. TNFR2 depletion reduces psoriatic inflammation in mice via downregulating specific dendritic cell populations in lymph nodes and inhibiting IL-23/IL-17 pathways Journal of Investigative Dermatology 8/1/2022 [PMID: 35090950]
Irina D. Pokrovskaya, Shilpi Yadav, Amith Rao, Emma McBride, Jeffrey A. Kamykowski, Guofeng Zhang et al. 3D ultrastructural analysis of alpha ‐granule, dense granule, mitochondria, and canalicular system arrangement in resting human platelets Research and Practice in Thrombosis and Haemostasis 1/1/2020 [PMID: 31989087]
Vitalyi Senyuk, Pritesh Patel, Nadim Mahmud, Damiano Rondelli Blockade of TNF alpha to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell Transplantation Frontiers in Immunology 1/22/2019 [PMID: 30723481]
K Ni, A Gill, D Cao, K Koike, KS Schweitzer, S Garantziot, I Petrache Intravascular heavy chain-modification of hyaluronan during endotoxic shock Biochem Biophys Rep, 2018-12-26;17(0):114-121. 2018-12-26 [PMID: 30623115] (Western Blot, Human)	Western Blot	Human
Jennifer Becker, Volker Kinast, Marius Döring, Christoph Lipps, Veronica Duran, Julia Spanier et al. Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines Virulence 12/31/2018 [PMID: 30403913]
M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer PLoS ONE, 2018-06-06;13(6):e0198347. 2018-06-06 [PMID: 29874279] (Neutralization, Human)	Neutralization	Human
V Rothhammer, DM Borucki, JE Kenison, P Hewson, Z Wang, R Bakshi, DH Sherr, FJ Quintana Detection of aryl hydrocarbon receptor agonists in human samples Sci Rep, 2018-03-21;8(1):4970. 2018-03-21 [PMID: 29563571] (Neutralization, Human)	Neutralization	Human
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339495] (Affinity Assay)	Affinity Assay
Eun Young Kim, Hila Roshanravan, Stuart E. Dryer Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 9/1/2017 [PMID: 28629718]
M A Rochat Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV J. Virol, 2017-01-31;0(0):. 2017-01-31 [PMID: 27928016] (Neutralization, Human)	Neutralization	Human
Tomone Nagae, Kiho Araki, Yuki Shimoda, Lucia I. Sue, Thomas G. Beach, Yoshihiro Konishi Cytokines and Cytokine Receptors Involved in the Pathogenesis of Alzheimer's Disease Journal of Clinical & Cellular Immunology 11/30/2016 [PMID: 27895978]
Xiaoliang Wang, Zhaoyue He, He Liu, Shida Yousefi, Hans-Uwe Simon Neutrophil Necroptosis Is Triggered by Ligation of Adhesion Molecules following GM-CSF Priming The Journal of Immunology 11/15/2016 [PMID: 27815445]
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016-09-20;11(9):e0162908. 2016-09-20 [PMID: 27649540] (Bioassay, Human)	Bioassay	Human
Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT. Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96. 2014-12-15 [PMID: 25512551] (Neutralization, Human)	Neutralization	Human
Todd J, Simpson P, Estis J, Torres V, Wub A Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine, 2013-10-12;64(3):660-5. 2013-10-12 [PMID: 24128872] (ELISA Development, Human)	ELISA Development	Human
Mizuki Yamamoto, Yuu Taguchi, Taku Ito-Kureha, Kentaro Semba, Noritaka Yamaguchi, Jun-ichiro Inoue NF-kappa B non-cell-autonomously regulates cancer stem cell populations in the basal-like breast cancer subtype Nature Communications 8/12/2013 [PMID: 23934482]
Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells. J Immunol, 2013-04-10;190(10):5321-8. 2013-04-10 [PMID: 23576682] (Neutralization, Human)	Neutralization	Human
M.M. Tang, Z. Spanou, H. Tang, F. Schibler, N. Pelivani, N. Yawalkar Rapid Downregulation of Innate Immune Cells, Interleukin-12 and Interleukin-23 in Generalized Pustular Psoriasis with Infliximab in Combination with Acitretin Dermatology 9/24/2012 [PMID: 23343611]
Sture Forsgren, Lina Renström, Craig Purdam, James E. Gaida TNF-Alpha in the Locomotor System beyond Joints: High Degree of Involvement in Myositis in a Rabbit Model International Journal of Rheumatology 3/7/2012 [PMID: 22505941]
Yaël Berda-Haddad, Stéphane Robert, Paul Salers, Leila Zekraoui, Catherine Farnarier, Charles A. Dinarello et al. Sterile inflammation of endothelial cell-derived apoptotic bodies is mediated by interleukin-1 alpha Proceedings of the National Academy of Sciences 12/20/2011 [PMID: 22143786]
Roman Fischer, Olaf Maier, Martin Siegemund, Harald Wajant, Peter Scheurich, Klaus Pfizenmaier A TNF Receptor 2 Selective Agonist Rescues Human Neurons from Oxidative Stress-Induced Cell Death PLoS ONE 11/14/2011 [PMID: 22110694]
Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. J Leukoc Biol, 2011-11-01;91(2):299-309. 2011-11-01 [PMID: 22045868] (Neutralization, Human)	Neutralization	Human
Maria Ângela B. Trindade, Noemia B Carvalho, Maria A. Shikanai-Yasuda, Erika Pagliari, Noemia B. Carvalho, Neusa Y Sakai-Valente et al. A Patient with Erythema Nodosus Leprosum and Chagas Cardiopathy: Challenges in Patient Management and Review of the Literature The American Journal of Tropical Medicine and Hygiene 6/3/2011 [PMID: 21633036]
Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma. Haematologica, 2011-05-23;0(0):. 2011-05-23 [PMID: 21606160] (Neutralization, Human)	Neutralization	Human
Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. J. Immunol., 2011-03-02;186(7):4175-82. 2011-03-02 [PMID: 21368235] (Neutralization, Human)	Neutralization	Human
Vera Luiza Capelozzi, Edwin Roger Parra, Manoel Ximenes, Ricardo Helbert Bammann, Carmen Silvia Valente Barbas, Marid Irmd Seixas Duarte Pathological and ultrastructural analysis of surgical lung biopsies in patients with swine-origin influenza type A/H1N1 and acute respiratory failure Clinics (Sao Paulo) 12/1/2010 [PMID: 21340209]
Hasegawa H, Thomas HJ, Schooley K, Born TL Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein. Cytokine, 2011-01-01;53(1):74-83. 2011-01-01 [PMID: 20926308] (Western Blot, Human)	Western Blot	Human
Christopher W. Espelin, Arthur Goldsipe, Peter K. Sorger, Douglas A. Lauffenburger, David de Graaf, Bart S. Hendriks Elevated GM-CSF and IL-1beta levels compromise the ability of p38 MAPK inhibitors to modulate TNFalpha levels in the human monocytic/macrophage U937 cell line Molecular BioSystems 7/10/2010 [PMID: 20617251]
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010-06-10;116(12):2061-9. 2010-06-10 [PMID: 20538796] (IHC, Human)	IHC	Human
Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease. J. Exp. Med., 2009-08-03;206(9):1883-97. 2009-08-03 [PMID: 19652016] (Western Blot, Human)	Western Blot	Human
Navenot JM, Fujii N, Peiper SC KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Mol. Pharmacol., 2009-02-06;75(5):1074-83. 2009-02-06 [PMID: 19201817] (Neutralization, Human)	Neutralization	Human
Audrey Luce, Aurélie Courtin, Céline Levalois, Sandrine Altmeyer-Morel, Paul-Henri Romeo, Sylvie Chevillard et al. Death receptor pathways mediate targeted and non-targeted effects of ionizing radiations in breast cancer cells Carcinogenesis 3/1/2009 [PMID: 19126655]
Skibinski G, Elborn JS, Ennis M Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76. 2007-03-23 [PMID: 17384084] (Neutralization, Human)	Neutralization	Human
Lee SY, Cherla RP, Tesh VL Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1. Infect. Immun., 2006-12-28;75(3):1291-302. 2006-12-28 [PMID: 17194804] (Functional Assay, Human)	Functional Assay	Human
Hunter I, Nixon GF Spatial compartmentalization of tumor necrosis factor (TNF) receptor 1-dependent signaling pathways in human airway smooth muscle cells. Lipid rafts are essential for TNF-alpha-mediated activation of RhoA but dispensable for the activation of the NF-kappaB and MAPK pathways. J. Biol. Chem., 2006-09-18;281(45):34705-15. 2006-09-18 [PMID: 16982613] (Neutralization, Human)	Neutralization	Human
Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S Sepsis and pathophysiology of anthrax in a nonhuman primate model. Am. J. Pathol., 2006-08-01;169(2):433-44. 2006-08-01 [PMID: 16877346] (ELISA Development, Primate - Papio cynocephalus (Yellow Baboon))	ELISA Development	Primate - Papio cynocephalus (Yellow Baboon)
Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. J. Leukoc. Biol., 2005-06-03;78(2):359-71. 2005-06-03 [PMID: 15937142] (Neutralization, Human)	Neutralization	Human
Andersson By U, Tani E, Andersson U Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis. J. Pediatr. Hematol. Oncol., 2004-11-01;26(11):706-11. 2004-11-01 [PMID: 15543003] (ICC, Human)	ICC	Human
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Photochem. Photobiol., 2004-09-01;80(2):231-5. 2004-09-01 [PMID: 15362934] (IHC, Human)	IHC	Human
Park JS, 107290, Kim YS, Jee YK, Myong NH, Lee KY Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta. Active immunotherapy against pathogenic Cis pT231-tau suppresses neurodegeneration in traumatic brain injury mouse models, 2003-05-01;57(5):463-9. 2003-05-01 [PMID: 12753503] (Neutralization, Human)	Neutralization	Human
Edgeworth JD, Spencer J, Phalipon A, Griffin GE, Sansonetti PJ Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells. Eur. J. Immunol., 2002-05-01;32(5):1464-71. 2002-05-01 [PMID: 11981835] (Western Blot, Human)	Western Blot	Human
Show All 41 Publications. Collapse Publications.

Catalog# & Formulation	Size	Price
Carrier Free AF-210-SP

Gene Symbol	TNF
Uniprot	P01375()