![Western Blot: TLR9 Antibody [NBP1-77254] - Mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-77254-img0005.jpg "Western Blot: TLR9 Antibody [NBP1-77254] - Mouse spleen cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
| Reactivity | Hu, Mu, Bv, ShSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human TLR9. The immunogen is located within amino acids 960 - 1010 of TLR9. Amino Acid Squence: LSPDGRRSRYVRLRQR |
| Predicted Species | Bovine (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TLR9 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NBP1-77254 | Applications | Species |
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| Trune DR, Kempton B, Hausman FA et al. Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media Hear. Res. 2015-01-01 [PMID: 25922207] (IHC-P, Mouse) | IHC-P | Mouse |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
Simple Western | Mouse | 08/11/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TLR9 Antibody (NBP1-77254)Find related products by research area.
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Toll-like receptors in the intestinal epithelial cells By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ... Read full blog post. |
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Topics in CD11b: The innate immune response Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens. Integrin... Read full blog post. |
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MHC Class I and the Herpes Simplex Virus MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens. These molecules are highly polymorphic, meaning that they exist in multiple varian... Read full blog post. |
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The role of TLR4 in breast cancer Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation. In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy... Read full blog post. |
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IRAK4: The "master IRAK" critical for initiating immune responses IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec... Read full blog post. |
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TLR9: For Whom the Cell Tolls The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found... Read full blog post. |
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TLR9: Tollgate to Immunity Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla... Read full blog post. |
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TLR9, Infectious Disease and Cancer Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen... Read full blog post. |
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TLR9 Antibodies in Immunity Research Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu... Read full blog post. |
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| Verified Customer 08/11/2015 |
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| Application: | Simple Western | |
| Species: | Mouse |
![Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-77254] - Mouse spleen cells 10 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77254-img0003.jpg "Immunocytochemistry/Immunofluorescence: TLR9 Antibody [NBP1-77254] - Mouse spleen cells 10 ug/ml.")
![Immunohistochemistry-Paraffin: TLR9 Antibody [NBP1-77254] - Mouse spleen cells with TLR9 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TLR9-Antibody-Immunohistochemistry-Paraffin-NBP1-77254-img0004.jpg "Immunohistochemistry-Paraffin: TLR9 Antibody [NBP1-77254] - Mouse spleen cells with TLR9 antibody at 2 ug/ml.")
![Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] - Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-77254_rabbit-polyclonal-tlr9-antibody-2711202420285895.jpg "Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] - Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 u/mL.")
![Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] - Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77254_rabbit-polyclonal-tlr9-antibody-2711202419201550.jpg "Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] - Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 u/mL.")
![Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] - Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77254_rabbit-polyclonal-tlr9-antibody-2711202420312921.jpg "Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-77254] - Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 u/mL.")
![Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] - Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp1-77254_rabbit-polyclonal-tlr9-antibody-2711202419293894.jpg "Immunohistochemistry: TLR9 Antibody - BSA Free [NBP1-77254] - Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 u/mL.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Agonist Activity CD40/TNFRSF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD40_AF632_Functional_Assay_15457.jpg)
![Western Blot CD40/TNFRSF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD40_AF632_Western_Blot_20204.jpg)
![Simple Western CD40/TNFRSF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/af632_human-cd40-tnfrsf5-affinity-purified-polyclonal-ab-simple-western-58202111562.jpg)
![CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](https://images.novusbio.com/images/CpG-oligodeoxynucleotides-with-negative-control--TLR9-ligand-NBP2-26232-img0002.jpg "CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
