TLR5 Antibody (19D759.2) - BSA Free

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 10 applications: Block/Neutralize, Cytometric Bead Assay Standard, FLOW, Flow Cytometry, Flow Cytometry Control, Flow-CS, ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 42. Show All 42 Publications. Collapse Publications.

Publications using NBP2-24787	Applications	Species
Qiu C, Wang J, Zhu L et al. Improving the ex vivo expansion of human tumor-reactive CD8 + T cells by targeting toll-like receptors Frontiers in Bioengineering and Biotechnology 2022-10-31 [PMID: 36394017] (Flow Cytometry, Block/Neutralize)	Flow Cytometry, Block/Neutralize
Kylma AK, Jouhi L, Listyarifah D et al. Treponema denticola chymotrypsin-like protease as associated with HPV-negative oropharyngeal squamous cell carcinoma. Br. J. Cancer. 2018-06-22 [PMID: 29930251] (IF/IHC, Human)	IF/IHC	Human
Sipola S. COLECTOMY IN AN ICU PATIENT POPULATION. Thesis 2014-01-01 (IHC-P, Human) Details: Normal and diseased colon, The TLR5 mAb was used at 1:50.	IHC-P	Human
Eskuri M, Kemi N, Helminen O et al. Toll-like receptors 1, 2, 4, 5, and 6 in gastric cancer Virchows Archiv : an international journal of pathology 2023-09-26 [PMID: 37750927]
Beilmann-Lehtonen I, HagstrOm J, Mustonen H et al. High Tissue TLR5 Expression Predicts Better Outcomes in Colorectal Cancer Patients Oncology 2021-06-17 [PMID: 34139707]
Kairaluoma V, Kemi N, Huhta H et al. Toll-like receptor 5 and 8 in hepatocellular carcinoma APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2021-05-05 [PMID: 33950532]
Kitaura A, Nishinaka T, Hamasaki S, et al. Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages PloS one 2021-01-25 [PMID: 33493233]
Shang L, Deng D, Roffel S, Gibbs S Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa Contact Derm. 2020-07-16 [PMID: 32677222] (WB, Human)	WB	Human
KylmA AK, Tolvanen TA, CarpEn T et al. Elevated TLR5 expression in vivo and loss of NF-kappa B activation via TLR5 in vitro detected in HPV-negative oropharyngeal squamous cell carcinoma Exp. Mol. Pathol. 2020-03-30 [PMID: 32240617] (IF/IHC, Human)	IF/IHC	Human
Kasurinen A, Hagstrom J, Laitinen A et al. Evaluation of toll-like receptors as prognostic biomarkers in gastric cancer: high tissue TLR5 predicts a better outcome Sci Rep [PMID: 31467388] (IHC-P, Human)	IHC-P	Human
Lanki M, Seppanen H, Mustonen H et al. Toll-like receptor 1 predicts favorable prognosis in pancreatic cancer PLoS ONE 2019-07-17 [PMID: 31314777]
Ruuskanen M, Leivo I, Minn H et al. Expression of toll-like receptors in non-endemic nasopharyngeal carcinoma BMC Cancer 2019-06-25 [PMID: 31238894] (IHC-P, Human)	IHC-P	Human
Haeggblom L, Nasman A, Ramqvist T et al. TLR5 and TLR7 are differentially expressed in human papillomavirus-positive and negative base of tongue squamous cell carcinoma, and TLR7 may have an independent prognostic influence Acta Otolaryngol. 2019-02-22 [PMID: 30794027] (IHC-P, Human)	IHC-P	Human
Makinen, LK. Matrix metalloproteinases and toll-like receptors in early-stage oral tongue squamous cell carcinoma J Oral Pathol Med 2018-05-11 [PMID: 29747237]
Na Bon Hyang, Hoang Thi Xoan, Kim Jae Young et al. Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-kappa B Activation in Human Myeloid Leukemia THP-1 Cells. BioMed Research International 2018-02-14 [PMID: 29651431] (FLOW, WB, Human)	FLOW, WB	Human
Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J Allergy Clin Immunol. [PMID: 28219705] (FLOW, Mouse) Details: Citation using the FITC form of this antibody.	FLOW	Mouse
Helminen O, Huhta H, Leppanen J et al. Nuclear localization of Toll-like receptor 5 in Barrett's esophagus and esophageal adenocarcinoma is associated with metastatic behavior Virchows Arch. 2016-07-09 [PMID: 27392931] (IF/IHC, Human)	IF/IHC	Human
Huhta H, Helminen O, Kauppila JH et al. The Expression of Toll-like Receptors in Normal Human and Murine Gastrointestinal Organs and the Effect of Microbiome and Cancer J. Histochem. Cytochem. 2016-08-01 [PMID: 27370795] (IHC-P, Human)	IHC-P	Human
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. 2016-07-05 [PMID: 27248820] (Cytometric Bead Assay Standard, Human)	Cytometric Bead Assay Standard	Human
Carevic M, Oz H, Fuchs K et al. CXCR1 Regulates Pulmonary Anti-Pseudomonas Host Defense. J Innate Immun. 2016-03-08 [PMID: 26950764] Details: This citation used the FITC version of this antibody.
Renkonen J, Toppila-Salmi S, Joenvaara S et al. Expression of Toll-like receptors in nasal epithelium in allergic rhinitis APMIS. 2015-06-08 [PMID: 26061394] (IHC-P, Human) Details: TLR7 antibody (Imgenex IMG-581A) was used for IHC-P on nasal biopsies from healthy non-smoking volunteers both allergic to birch pollen and non-allergic controls. Nasal biopsies were collected before and after off-seasonal intranasal birch pollen or diluent challenge and the immunostaining assay implicated - 4um paraffin sections, antigen retrieval withTris-HCl pH 8.5, processing on Autostainer, use of primary at 1.67 ug/mL concentration, Rabbit/Mouse REAL EnVision Detection system and Peroxidase/DAB+ detection (Fig 1, Fig 2D ).	IHC-P	Human
Jouhi L, Datta N, Renkonen S et al. Expression of toll-like receptors in HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma-an in vivo and in vitro study. Tumour Biol. 2015-05-05 [PMID: 25941114] (IHC-P, Human) Details: TLR5 antibody was used for IHC-P analysis of formalin-fixed paraffin-embedded sections of human HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma tissues.	IHC-P	Human
Makinen LK, Atula T, Hayry V et al. Predictive role of toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2014-09-25 [PMID: 25264223] (IHC-P, Human) Details: TLR7 antibody used at 1:300 dilution for IHC-P on oral tongue squamous cell carcinoma tissues / OTSCC (Stage I-II patients) - TMA paraffin sections, antigen retrieval with PreTreatment-module in Tris-HCl buffer pH 8.5 at 98C for 20 min, Autostainer based assay with 1 hour primary incubation time followed by detection using REAL EnVision/HRP-DAB system (Fig 1, Nuclear membranous staining pattern).	IHC-P	Human
Uchida M, Oyanagi E, Kawanishi N et al. Exhaustive exercise increases the TNF-a production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice Immunol Lett 2014-03-03 [PMID: 24412598] (Flow Cytometry Control, Flow-CS, IHC-P, Mouse, Human) Details: This citation used the FITC version of this antibody.	Flow Cytometry Control, Flow-CS, IHC-P	Mouse, Human
Helminen O, Huhta H, Takala H et al. Increased Toll-like receptor 5 expression indicates esophageal columnar dysplasia. Virchows Arch 2013-11-13 [PMID: 24221343] (IHC-P, Human)	IHC-P	Human
Grimm M, Munz A, Exarchou A et al. Immunohistochemical detection of Helicobacter pylori without association of TLR5 expression in oral squamous cell carcinoma. J. Oral Pathol. Med. 2014-01-01 [PMID: 23659788] (ICC/IF, WB, IHC-P, Human) Details: IHC (P): oral squamouse cell carcinoma (Figs 2 & 3, Table 2); ICC: BIRC3 and BICR56 oral squamous call carcinoma (OSCC) cell lines (Fig 4A), WB: BIRC3, BICR56, normal human mucosa (Fig 4C,D. TLR5 was detected just below the 100 kDa marker. TLR5 was detect	ICC/IF, WB, IHC-P	Human
Kauppila JH, Mattila AE, Karttunen TJ, Salo T. Toll-like receptor 5 (TLR5) expression is a novel predictive marker for recurrence and survival in squamous cell carcinoma of the tongue. Br J Cancer. 2013-02-19 [PMID: 23287987] (IF/IHC) Details: TLR5 (IMG-664A, Clone 19D759.2) IHC in normal epithelium and squamous cell carcinoma of the tongue, Figs 1, 3.	IF/IHC
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897] (Flow-CS, Human) Details: Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (	Flow-CS	Human
Kato K, Lillehoj EP, Park YS et al. Membrane-tethered MUC1 mucin is phosphorylated by epidermal growth factor receptor in airway epithelial cells and associates with TLR5 to inhibit recruitment of MyD88. J Immunol. 2012-02-15 [PMID: 22250084] (WB, Human) Details: Product cited: TLR5 (IMG-664A). WB: Mouse embryonic fibroblasts: Fig 1A. Human embryonic kidney cells, Figs 3A, 3C, 4A, & 4B. Human lung epithelial cells, primary mouse tracheal surface epithelial cells, human tracheal epithelial cells, Fig 5A. Human embr	WB	Human
Faham A, Altin JG. Antigen-containing liposomes engrafted with flagellin-related peptides are effective vaccines that can induce potent antitumor immunity and immunotherapeutic effect. J Immunol. 2010-08-01 [PMID: 20610649] Details: Product cited: TLR5 Alexa Fluor (IMG-664AF488): Flow (cell surface): HEK-293, HepG2, CHO, and BMDCs (bone marrow derived dendridic cells) cell lines, Fig 3. Note: Cell surface expression of TLR5 was found to be relativetly strong in HEK-293 and moderate i
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426] Details: Citation using the PE/Cy5 form of this antibody.
Deng J, Ma-Krupa W, Gewirtz AT et al. Toll-like receptors 4 and 5 induce distinct types of vasculitis. Circ Res. 2009-02-27 [PMID: 19150884] (IHC-P, Human) Details: IMG-664A: IHC (paraffin), human temporal arteries, Fig. 1E,G.	IHC-P	Human
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] Details: Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Fukata M, Breglio K, Chen A et al. The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease. J Immunol. 2008-02-01 [PMID: 18209086] (Flow-CS, Flow Cytometry Control, Mouse) Details: Flow (Cell Surface) + Flow (Intracellular) [mouse splenic CD4+CD45Rbhigh T cells, RAW 264.7 cells], Fig. 1. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Burgener IA and TW Jungi. Veterinary Immuno	Flow-CS, Flow Cytometry Control	Mouse
Ha H, Lee JH, Kim HN et al. Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta. J Immunol. 2008-02-01 [PMID: 18209032] (WB, Mouse) Details: WB (mouse osteoclasts and bone marrow cells), Fig. 1A.	WB	Mouse
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
Crellin NK Garcia RV Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. [PMID: 16339542] Details: Citation using the Azide and Endotoxin Free form of this antibody.
Miller LS, SOrensen OE, Liu PT et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005-05-15 [PMID: 15879109]
Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol [PMID: 15749901] (WB) Details: Used the FITC form of this antibody.	WB
Lanki M Prognostic and Differential Diagnostic Biomarkers in Pancreatic Ductal Adenocarcinoma Thesis 2023-01-01
Uchida M, Oyanagi E, Kawanishi N et al. Exhaustive exercise increases the TNF-a production in response to flagellin via the upregulation of toll-like receptor 5 in the large intestine in mice Immunol Lett (IHC-P, Flow-CS, FLOW, Human, Mouse) Details: This publication used the FITC conjugated form of this antibody (Cat# NBP2-24784).	IHC-P, Flow-CS, FLOW	Human, Mouse
Smith Nicholas J, Varley Claire L, Eardley Ian et al. Toll-like receptor responses of normal human urothelial cells to bacterial flagellin and lipopolysaccharide. J Urol. 2011-09-01 [PMID: 21784459]
Show All 42 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Monica Sauter	IHC-P	maccaca mulatta	04/06/2018	View Summary Application Immunohistochemistry-Paraffin Sample Tested formalin fixed paraffin embedded macaque retina tissue Species maccaca mulatta Lot CJYR01 Comments Comments heat mediated antigen retrieval (pH 6 citrate buffer) 1:50 dilution of antibody, overnight at 4C goat anti-mouse AlexaFluor 488 secondary antibody (1:400, 1 hr RT)

Array NBP2-24787

• TLR5 Antibodies
• TLR5 ELISA Kits
• TLR5 Lysate
• TLR5 Protein
• TLR5 Proteins and Enzyme