TLR5 Antibody (19D759.2) [PE]

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NBP2-24783	Applications	Species
Kauppila JH, Mattila AE, Karttunen TJ, Salo T. Toll-like receptor 5 (TLR5) expression is a novel predictive marker for recurrence and survival in squamous cell carcinoma of the tongue. Br J Cancer. 2013-02-19 [PMID: 23287987] (IF/IHC) Details: TLR5 (IMG-664A, Clone 19D759.2) IHC in normal epithelium and squamous cell carcinoma of the tongue, Figs 1, 3.	IF/IHC
Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897] Details: Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
Faham A, Altin JG. Antigen-containing liposomes engrafted with flagellin-related peptides are effective vaccines that can induce potent antitumor immunity and immunotherapeutic effect. J Immunol. 2010-08-01 [PMID: 20610649] Details: Product cited: TLR5 Alexa Fluor (IMG-664AF488): Flow (cell surface): HEK-293, HepG2, CHO, and BMDCs (bone marrow derived dendridic cells) cell lines, Fig 3. Note: Cell surface expression of TLR5 was found to be relativetly strong in HEK-293 and moderate i
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426] Details: Citation using the PE/Cy5 form of this antibody.
Deng J, Ma-Krupa W, Gewirtz AT et al. Toll-like receptors 4 and 5 induce distinct types of vasculitis. Circ Res. 2009-02-27 [PMID: 19150884] (IHC-P, Human) Details: IMG-664A: IHC (paraffin), human temporal arteries, Fig. 1E,G.	IHC-P	Human
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] Details: Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Fukata M, Breglio K, Chen A et al. The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease. J Immunol. 2008-02-01 [PMID: 18209086] Details: Flow (Cell Surface) + Flow (Intracellular) [mouse splenic CD4+CD45Rbhigh T cells, RAW 264.7 cells], Fig. 1. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Burgener IA and TW Jungi. Veterinary Immuno
Ha H, Lee JH, Kim HN et al. Stimulation by TLR5 modulates osteoclast differentiation through STAT1/IFN-beta. J Immunol. 2008-02-01 [PMID: 18209032] (WB, Mouse) Details: WB (mouse osteoclasts and bone marrow cells), Fig. 1A.	WB	Mouse
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
Crellin NK Garcia RV Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. [PMID: 16339542] Details: Citation using the Azide and Endotoxin Free form of this antibody.
Miller LS, SOrensen OE, Liu PT et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005-05-15 [PMID: 15879109]
Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol [PMID: 15749901] (WB) Details: Used the FITC form of this antibody.	WB
Show All 12 Publications. Collapse Publications.

Array NBP2-24783

• TLR5 Antibodies
• TLR5 ELISA Kits
• TLR5 Lysate
• TLR5 Protein
• TLR5 Proteins and Enzyme