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TLR5 Antibody (624915) [Unconjugated]

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TLR5 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panels show a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TLR5 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Human Human Peripheral Blood Monocytes were stained with (A) Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
624915
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6704U-100UG
Alexa Fluor 405
FAB6704V-100UG
Alexa Fluor 488
FAB6704G
Alexa Fluor 532
FAB6704X
Alexa Fluor 594
FAB6704T-100UG
Alexa Fluor 647
FAB6704R-100UG
Alexa Fluor 700
FAB6704N-100UG
Alexa Fluor 750
FAB6704S-100UG
Allophycocyanin
FAB6704A
Allophycocyanin/Cy7
FAB6704APCCY7
Biotin
FAB6704B
CoraFluor 1
FAB6704CL1
DyLight 350
FAB6704D
DyLight 405
FAB6704E
DyLight 488
FAB6704K
DyLight 550
FAB6704L
DyLight 594
FAB6704M
DyLight 650
FAB6704W
DyLight 680
FAB6704Y
DyLight 755
FAB6704Z
FITC
FAB6704F
HRP
FAB6704H
Janelia Fluor 525
FAB6704JF525
Janelia Fluor 549
FAB6704I
Janelia Fluor 646
FAB6704J
Janelia Fluor 669
FAB6704JF669
PE
FAB6704P
PE/Atto594
FAB6704PEATT594
PE/Cy5.5
FAB6704PECY55
PE/Cy7
FAB6704PECY7
PerCP
FAB6704C
mFluor Violet 450 SE
FAB6704MFV450
mFluor Violet 500 SE
FAB6704MFV500
mFluor Violet 610 SE
FAB6704MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB6704G
Carrier Free
MAB6704-SP
Carrier Free
FAB6704V-100UG
Carrier Free
FAB6704U-100UG
Carrier Free
FAB6704T-100UG
Carrier Free
FAB6704S-100UG
Carrier Free
FAB6704R-100UG
Carrier Free
FAB6704N-100UG
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TLR5 Antibody (624915) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TLR5
Ile21-Phe115
Accession # O60602
Specificity
Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB6704 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR5 Antibody (624915) [Unconjugated]

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Background

TLR5 is an ~100 kDa type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% amino acid identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR5 Antibody (MAB6704)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TLR5
Uniprot