TLR2 Antibody - Azide Free

Images

 
Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.
Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml. Image using the standard format of this product.
Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide Free
Concentration
1.0 mg/ml

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TLR2 Antibody - Azide Free Summary

Immunogen
This antibody was developed against a mixture of synthetic peptides containing amino acids 180-196, 353-370, and 473-489 of human TLR2 (NP_003255).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Western Blot 1-3 ug/ml
Application Notes
In transfected human TLR2 cell lysate, a 90 kDa band is observed. This has also been reported to work well in bronchial epithelial cells (UBH16E) and THP-1 cells.
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Antagonist
Publications
Read Publications using
NBP2-24861 in the following applications:

  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml sterile PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR2 Antibody - Azide Free

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR2 Antibody (NBP2-24861)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP2-24861 Applications Species
Millar FR, Pennycuick A, Muir M Et al. Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer Cell Rep 2022-11-09 [PMID: 36351380] (IHC-P, Mouse, Human)

Details:
Citation using the Azide Free version of this antibody.
IHC-P Mouse, Human
Su X, Ao L, Shi Y et al. Oxidized low density lipoprotein induces bone morphogenetic protein-2 in coronary artery endothelial cells via Toll-like receptors 2 and 4. J Biol Chem. 2011-04-08 [PMID: 21325271] (Human)

Details:
TLR2/CD282 (IMG-410A). WB: TLR2 siRNA transfected human coronary artery endothelial (CAECs) cell line, Fig 3A. Note: The TLR2 (IMG-410A) antibody was siRNA validated by WB in CAEC cell line, Fig 3A.
Human
Ghanim H, Sia CL, Upadhyay M et al. Orange juice neutralizes the proinflammatory effect of a high-fat, high-carbohydrate meal and prevents endotoxin increase and Toll-like receptor expression. Am J Clin Nutr. 2010-04-01 [PMID: 20200256]
Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008-09-01 [PMID: 18499758] (WB)

Details:
Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
WB
Chun J, Prince A. Activation of Ca2+-dependent signaling by TLR2. J Immunol. 2006-07-15 [PMID: 16818794] (WB, Human)

Details:
1. TLR2 (IMG-410) [WB (human airway epithelial cell lines)].
WB Human
LeBouder E, Rey-Nores JE, Rushmere NK et al. Soluble forms of Toll-like receptor (TLR)2 capable of modulating TLR2 signaling are present in human plasma and breast milk. J Immunol. 2003-12-15 [PMID: 14662871] (WB, Human)

Details:
1. TLR2 (IMG-319 and IMG-410); For IMG-319: WB, Fig.1D and Fig.6C (Mono Mac-6 monocytes); For IMG-410: Fig.6A-B, (human breast milk).
WB Human

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional TLR2 Products

Research Areas for TLR2 Antibody (NBP2-24861)

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Blogs on TLR2. Showing 1-10 of 11 blog posts - Show all blog posts.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.


  Read full blog post.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol TLR2