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MALP-2, TLR6 and TLR2 ligand

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MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells ...read more
MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like ...read more
MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Differentiated human macrophages were treated with or without MALP-2 (100 ng/mL), and the release of IL-6 in culture supernatants of cells was measured by ELISA kit. Image ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

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MALP-2, TLR6 and TLR2 ligand Summary

Description
Disclaimer Note -Disclaimer Note - Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits. Human Serum- No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Immunogen
MALP-2, TLR6 and TLR2 ligand
Specificity
MALP-2 was originally isolated from Mycoplasma fermentans. This MALP-2 corresponds to the originally isolated isomer, which expresses potent endotoxin-like activity and approaches in certain experimental systems the toxicity of LPS. For description of the stereochemistry of MALP-2 please refer to M. Morr, et al. Eur. J. Immunol. 32, 3337 (2002). The importance of the stereochemistry of the central carbon atom of the diacylglycerol group has been described in the K.M. Omueti, et al. paper (2005), see below. Formula: C99H167N19O30S
Details of Functionality
Specific Activity is approx 2x10^8units/mg. One unit is defined as the dilution giving half maximal release of nitric oxide from C3H/HeJ mouse peritoneal exudate cells in the standard assay.

Applications/Dilutions

Dilutions
  • Functional
  • In vitro assay
Application Notes
Source: Synthetic
Formula: C99H167N19O30S
MW: 2135.2
Use in In-vitro and functional assay reported in scientific literature (PMID: 25466255).
Reviewed Applications
Read 1 Review rated 5
using
NBP2-26219 in the following applications:

Publications
Read Publications using
NBP2-26219 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
2 ug in 20 uL of sterile PBS solution containing 2.5% (v/v) 2-propanol, 25 mM n-octyl-b-D-glucopyranoside and 1% (wt/v) human serum albumin.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for MALP-2, TLR6 and TLR2 ligand

  • Macrophage-activating lipopeptide-2
  • MALP 2
  • MALP-2, TLR6 & TLR2 ligand
  • S-[2,3-bis(Palmityloxy)-(2R)-propyl-cysteinyl-GNNDESNISFKEK]
  • TLR activator MALP2

Background

MALP-2, TLR6 and TLR2 ligand sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2, TLR6 and TLR2 ligand stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2, TLR6 and TLR2 ligand is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for MALP-2, TLR6 and TLR2 ligand (NBP2-26219)(19)

We have publications tested in 4 confirmed species: Human, Mouse, Avian, Bacteria.

We have publications tested in 9 applications: Block/Neutralize, FLOW, Func, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro, In-vitro, LA, Western Blot.


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Block/Neutralize
(1)
FLOW
(1)
Func
(6)
Immunocytochemistry/ Immunofluorescence
(1)
Immunoprecipitation
(1)
In vitro
(5)
In-vitro
(1)
LA
(1)
Western Blot
(1)
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Human
(7)
Mouse
(4)
Avian
(1)
Bacteria
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP2-26219 Applications Species
Liu Y, Diamond SL. Activation of Most Toll-Like Receptors in Whole Human Blood Attenuates Platelet Deposition on Collagen under Flow Journal of Immunology Research 2023-01-17 [PMID: 36703865] (Block/Neutralize) Block/Neutralize
Calses PC, Pham VC, Guarnaccia AD et al. TEAD Proteins Associate With DNA Repair Proteins to Facilitate Cellular Recovery From DNA Damage Molecular & Cellular Proteomics 2023-02-01 [PMID: 36640924] (Immunoprecipitation, Western Blot, Immunocytochemistry/ Immunofluorescence) Immunoprecipitation, Western Blot, Immunocytochemistry/ Immunofluorescence
Gasiorek A, Dobosz E, Potempa B Et Al. Subversion of Lipopolysaccharide Signaling in Gingival Keratinocytes via MCPIP-1 Degradation as a Novel Pathogenic Strategy of Inflammophilic Pathobionts mBio 2021-06-29 [PMID: 34182783]
Nogiec A, Bzowska M, Demczuk A Phenotype and Response to PAMPs of Human Monocyte-Derived Foam Cells Obtained by Long-Term Culture in the Presence of oxLDLs Front Immunol 2020-08-04 [PMID: 32849539] (Bacteria) Bacteria
Maruyama K, Kidoya H, Takemura N et al. Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages Cell Rep 2020-07-14 [PMID: 32668247]
Kanoh H, Nitta T, Go S et al. Homeostatic and pathogenic roles of GM3 ganglioside molecular species in TLR4 signaling in obesity EMBO J. 2020-05-07 [PMID: 32378734] (Mouse) Mouse
Bi J, Koivisto L, Pang A et al. Suppression of alphavB6 Integrin Expression by Polymicrobial Oral Biofilms in Gingival Epithelial Cells Sci Rep 2017-06-30 [PMID: 28667248] (In vitro, Human) In vitro Human
Battin C, Hennig A, Mayrhofer P et al. A human monocytic NF-kB fluorescent reporter cell line for detection of microbial contaminants in biological samples. PLoS ONE 2017-05-25 [PMID: 28542462] (FLOW, Human) FLOW Human
Yanai S, Tokuhara D, Tachibana D et al. Diabetic pregnancy activates the innate immune response through TLR5 or TLR1/2 on neonatal monocyte J. Reprod. Immunol. 2016-06-22 [PMID: 27351455] (In vitro, Human) In vitro Human
Sigola LB, Fuentes AL, Millis LM et al. Effects of toll-Like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis. Tissue and Cell. 2016-04-22 [PMID: 27157550] (Func, Mouse) Func Mouse
Show All 19 Publications.

Review for MALP-2, TLR6 and TLR2 ligand (NBP2-26219) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-26219:
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  5
reviewed by:
Eric Wang
06/21/2021
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LotL22330

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