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TIMP-2 Antibody [Unconjugated]

 (14 Publications)
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Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 ...read more
TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human TIMP-2 knockout HeLa human cervical epithelial carcinoma cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Recombinant Human MMP‑2 (0.2 µg/mL, Catalog #902‑MPN or 902‑MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971‑TM) that has been preincubated with increasing ...read more
Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for TIMP2 mRNA (ACD RNAScope Probe, catalog #470358; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, B/N, Dual ISH-IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF971
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF971-SP
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TIMP-2 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Cys27-Pro220
Accession # P16035
Specificity
Detects human and mouse TIMP‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human TIMP-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMP2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated 1 ug/mL
  • Neutralization 2.6 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF971 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-2 Antibody [Unconjugated]

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIMP-2 Antibody (AF971)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Functional Assay, IHC, IHC-Fr, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Functional Assay
(1)
IHC
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Neutralization
(2)
Western Blot
(4)
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Human
(6)
Mouse
(2)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF971 Applications Species
Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A; Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells International journal of molecular sciences 2023-11-28 [PMID: 38069171] (Western Blot, Human) Western Blot Human
Xu L, Nirwane A, Xu T et al. Fibroblasts repair blood-brain barrier damage and hemorrhagic brain injury via TIMP2 Cell reports 11/22/2022 [PMID: 36417884]
Vivian C. Chioma, Anna Kruyer, Ana-Clara Bobadilla, Ariana Angelis, Zachary Ellison, Ritchy Hodebourg et al. Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens Cells Biological Psychiatry 5/1/2021 [PMID: 33579535]
Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis Frontiers in Oncology 8/25/2020 [PMID: 32984024]
Baker-Williams AJ, Hashmi F, Budzy?ski MA et al. Co-chaperones TIMP2 and AHA1 Competitively Regulate Extracellular HSP90:Client MMP2 Activity and Matrix Proteolysis Cell Reports 8/13/2019 [PMID: 31412254]
Baker-Williams AJ, Hashmi F, Budzy?ski MA et al. Co-chaperones TIMP2 and AHA1 Competitively Regulate Extracellular HSP90:Client MMP2 Activity and Matrix Proteolysis Cell Reports 2019-08-13 [PMID: 31412254]
D Lu, Y Liao, SH Zhu, QC Chen, DM Xie, JJ Liao, X Feng, MH Jiang, W He Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction Stem Cell Res Ther, 2019-04-27;10(1):127. 2019-04-27 [PMID: 31029167] (Neutralization, Mouse) Neutralization Mouse
JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray Human umbilical cord plasma proteins revitalize hippocampal function in aged mice Nature, 2017-04-19;544(7651):488-492. 2017-04-19 [PMID: 28424512] (Functional Assay, IHC-Fr, Immunoprecipitation, Neutralization, Human, Mouse) Functional Assay, IHC-Fr, Immunoprecipitation, Neutralization Human, Mouse
Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al. Unique Transcriptional Programs Identify Subtypes of AKI Journal of the American Society of Nephrology 6/1/2017 [PMID: 28028135]
Lin T, Yang S, Chiu C, Su H, Voon W, Chai C, Lai W, Sheu S Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcome. PLoS ONE, 2014-01-27;9(1):e86287. 2014-01-27 [PMID: 24475101] (IHC, Human) IHC Human
Show All 14 Publications.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TIMP2
Uniprot