![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroid Peroxidase Mouse Monoclonal Antibody (TPO/1922).](http://images.novusbio.com/fullsize/Thyroid-Peroxidase-Antibody-TPO-1922-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79923-img0001.jpg "Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroid Peroxidase Mouse Monoclonal Antibody (TPO/1922).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | TPO/1922 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79789). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment of human Thyroid Peroxidase (around aa 685-804) (Exact sequence is proprietary) (Uniprot: P07202) |
| Localization | Cytoplasmic, Cell Surface |
| Marker | Thyroid Marker |
| Specificity | Thyroperoxidase (TPO) is a 933-amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TPO |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Thyroid Peroxidase Antibody (NBP2-79923)Find related products by research area.
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| Gene Symbol | TPO |
![Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAbto protein X is equal to 29.](http://images.novusbio.com/fullsize/Thyroid-Peroxidase-Antibody-TPO-1922-Azide-and-BSA-Free-Protein-Array-NBP2-79923-img0002.jpg "Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAbto protein X is equal to 29.")
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