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TGF-beta 1 Antibody (OTI1E12) [PE/Cy5.5]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI1E12
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74495AF350
Alexa Fluor 405
NBP2-74495AF405
Alexa Fluor 488
NBP2-74495AF488
Alexa Fluor 532
NBP2-74495AF532
Alexa Fluor 594
NBP2-74495AF594
Alexa Fluor 647
NBP2-74495AF647
Alexa Fluor 700
NBP2-74495AF700
Alexa Fluor 750
NBP2-74495AF750
Allophycocyanin
NBP2-74495APC
Allophycocyanin/Cy7
NBP2-74495APCCY7
Biotin
NBP2-74495B
CoraFluor 1
NBP2-74495CL1
DyLight 350
NBP2-74495UV
DyLight 405
NBP2-74495V
DyLight 488
NBP2-74495G
DyLight 550
NBP2-74495R
DyLight 594
NBP2-74495DL594
DyLight 650
NBP2-74495C
DyLight 680
NBP2-74495FR
DyLight 755
NBP2-74495IR
FITC
NBP2-74495F
HRP
NBP2-74495H
Janelia Fluor 525
NBP2-74495JF525
Janelia Fluor 549
NBP2-74495JF549
Janelia Fluor 646
NBP2-74495JF646
Janelia Fluor 669
NBP2-74495JF669
PE
NBP2-74495PE
PE/Atto594
NBP2-74495PEATT594
PE/Cy7
NBP2-74495PECY7
PerCP
NBP2-74495PCP
Unconjugated
NBP2-46108
mFluor Violet 450 SE
NBP2-74495MFV450
mFluor Violet 500 SE
NBP2-74495MFV500
mFluor Violet 610 SE
NBP2-74495MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46108
Azide and BSA Free
NBP2-74495
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TGF-beta 1 Antibody (OTI1E12) [PE/Cy5.5] Summary

Immunogen
Full length recombinant protein of human TGF-beta 1 (NP_000651) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TGFB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TGF-beta 1 Antibody (OTI1E12) [PE/Cy5.5]

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGF beta
  • TGF beta1
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TGF-beta 1
Antibody (OTI1E12) [P ...
NBP2-74495PECY55
TGF-beta 1 Antibody (OTI1E12) [PE/Cy5.5]
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta ...  Read full blog post.
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Bioinformatics

Gene Symbol TGFB1