![Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-MEM-189-Flow-Cytometry-NB500-493-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IP, CyTOF-ready |
| Clone | MEM-189 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone MEM-189 was used by HLDA to establish CD designation. |
| Immunogen | KG1 human acute myelogenous leukaemia cell line (NM_001128148.1). |
| Localization | Cell membrane; Single-pass type II membrane protein. Melanosome (identified by mass spectrometry in melanosome fractions from stage I to stage IV). Transferrin receptor protein 1, serum form: Secreted. |
| Marker | Recycling Endosome Marker |
| Specificity | The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. HLDA VI; WS Code NL N-L025 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFRC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is useful for Western Blot analysis (non-reducing), immunoprecipitation, and flow cytometry. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready. |
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| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS (pH 8.0) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
| Publications using NB500-493 | Applications | Species |
|---|---|---|
| Kishimoto T et al. Leucocyte typing VI. Garl Publishing Inc. 1997-01-01 | ||
| Burgio F, Gaiser C, Brady K et al. A Perfused In Vitro Human iPSC-Derived Blood-Brain Barrier Faithfully Mimics Transferrin Receptor-Mediated Transcytosis of Therapeutic Antibodies Cellular and molecular neurobiology 2023-09-12 [PMID: 37698826] | ||
| Deng, K;Lu, Y;Finnema, SJ;Vangjeli, K;Huang, J;Huang, L;Goodearl, A; Application of In vitro transcytosis models to brain targeted biologics PloS one 2023-08-23 [PMID: 37611031] (In vivo assay) | In vivo assay |
Secondary Antibodies |
Isotype Controls |
Research Areas for TfR (Transferrin R) Antibody (NB500-493)Find related products by research area.
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Transferrin and the blood brain barrier Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i... Read full blog post. |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")