Immunohistochemistry-Paraffin: TFF3 Antibody [NBP1-76514] - in human small intestine tissue with TFF3 antibody at 2.5 ug/mL.
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 ug/mL.
Western Blot: TFF3 Antibody - BSA Free [NBP1-76514] - Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: TFF3 Antibody - BSA Free [NBP1-76514] - Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 ug/ml. Red: TFF3 Antibody Blue: DAPI staining
Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of TFF3. Amino Acid Squence: ECNNRGCCFDSRIPGV
Specificity
TFF3 antibody is predicted to not cross-react with other TFF family members.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TFF3
Purity
Peptide affinity purified
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11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (81%), Pig (81%).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for TFF3 Antibody - BSA Free
HITF, human intestinal trefoil factor10Intestinal trefoil factor
ITF
TFF3
TFI
trefoil factor 3 (intestinal)
Background
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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