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TCF4 Antibody (TCF4/2252)

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Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. ...read more
Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/2252) [NBP3-13789] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC
Clone
TCF4/2252
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14192AF350
Alexa Fluor 405
NBP3-14192AF405
Alexa Fluor 488
NBP3-14192AF488
Alexa Fluor 532
NBP3-14192AF532
Alexa Fluor 594
NBP3-14192AF594
Alexa Fluor 647
NBP3-14192AF647
Alexa Fluor 700
NBP3-14192AF700
Alexa Fluor 750
NBP3-14192AF750
Allophycocyanin
NBP3-14192APC
Biotin
NBP3-14192B
CoraFluor 1
NBP3-14192CL1
DyLight 350
NBP3-14192UV
DyLight 405
NBP3-14192V
DyLight 488
NBP3-14192G
DyLight 550
NBP3-14192R
DyLight 594
NBP3-14192DL594
DyLight 650
NBP3-14192C
DyLight 680
NBP3-14192FR
DyLight 755
NBP3-14192IR
FITC
NBP3-14192F
HRP
NBP3-14192H
Janelia Fluor 525
NBP3-14192JF525
Janelia Fluor 549
NBP3-14192JF549
Janelia Fluor 646
NBP3-14192JF646
Janelia Fluor 669
NBP3-14192JF669
PE
NBP3-14192PE
PerCP
NBP3-14192PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14192
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TCF4 Antibody (TCF4/2252) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14192)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary) (Uniprot: P15884 )
Localization
Nucleus, Cytoplasm
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TCF4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TCF4 Antibody (TCF4/2252)

  • BHLHB19
  • bHLHb19E2-2
  • Class B basic helix-loop-helix protein 19
  • Immunoglobulin transcription factor 2
  • ITF-2
  • ITF2PTHS
  • MGC149724
  • SEF-2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • SEF2MGC149723
  • SL3-3 enhancer factor 2
  • TCF-4
  • transcription factor 4

Background

TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TCF4
Uniprot