![Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61998-img0004.jpg "Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence AKSNGENGPRAPA-C, from the N Terminus of the protein sequence according to NP_114143; NP_001191169.1. |
| Epitope | AKSNGENGPRAPA-C |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TBC1D10A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ plasma membrane and nucleus of MCF7 cells. Flow Cytometry: Flow cytometric analysis of HEK293 cells. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TBC1D10A |
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP2-61998-img0002.jpg "Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.")
![Flow Cytometry: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Flow-Cytometry-NBP2-61998-img0003.jpg "Flow Cytometry: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP2-61998-img0001.jpg "Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.")
