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![Western Blot: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPI64 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP2-36551-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPI64 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated EPI64, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunoprecipitation-NBP2-36551-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61998-img0004.jpg "Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).")
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP2-61998-img0002.jpg)
![Flow Cytometry: TBC1D10A Antibody [NBP2-61998] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Flow-Cytometry-NBP2-61998-img0003.jpg)
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP2-61998] - (4.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP2-61998-img0001.jpg)
![Western Blot: TBC1D10A Antibody [NBP1-89294] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP1-89294-img0005.jpg "Western Blot: TBC1D10A Antibody [NBP1-89294] - Analysis in human cell line U-138MG.")
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP1-89294] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP1-89294-img0009.jpg)
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP1-89294] - Staining of human cervix shows strong granular cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP1-89294-img0006.jpg)
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP1-89294] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP1-89294-img0007.jpg)
![Immunohistochemistry-Paraffin: TBC1D10A Antibody [NBP1-89294] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunohistochemistry-Paraffin-NBP1-89294-img0008.jpg)
![Western Blot: TBC1D10A Antibody [NBP2-58911] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP2-58911-img0002.jpg "Western Blot: TBC1D10A Antibody [NBP2-58911] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.")
![Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-58911] - Staining of human cell line RT4 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58911-img0001.jpg)
![Western Blot: TBC1D10A Antibody [NBP1-79842] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP1-79842-img0002.jpg "Western Blot: TBC1D10A Antibody [NBP1-79842] - Jurkat cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: TBC1D10A Antibody [NBP1-79842] - Human sperm extract.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP1-79842-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-55894] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55894-img0002.jpg "Immunocytochemistry/Immunofluorescence: TBC1D10A Antibody [NBP2-55894] - Staining of human cell line SK-MEL-30 shows localization to plasma membrane.")
![Western Blot: TBC1D10A Antibody [NBP1-79843] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/TBC1D10A-Antibody-Western-Blot-NBP1-79843-img0002.jpg "Western Blot: TBC1D10A Antibody [NBP1-79843] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")