Home » TBC1D4 » TBC1D4 Antibodies » TBC1D4 Antibody (OTI5E6) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

TBC1D4 Antibody (OTI5E6) - Azide and BSA Free

 Submit a Review

Images

 
(
Enlarge)
Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TBC1D4 ...read more
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI5E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74462AF350
Alexa Fluor 405
NBP2-74462AF405
Alexa Fluor 488
NBP2-74462AF488
Alexa Fluor 532
NBP2-74462AF532
Alexa Fluor 594
NBP2-74462AF594
Alexa Fluor 647
NBP2-74462AF647
Alexa Fluor 700
NBP2-74462AF700
Alexa Fluor 750
NBP2-74462AF750
Allophycocyanin
NBP2-74462APC
Allophycocyanin/Cy7
NBP2-74462APCCY7
Biotin
NBP2-74462B
CoraFluor 1
NBP2-74462CL1
DyLight 350
NBP2-74462UV
DyLight 405
NBP2-74462V
DyLight 488
NBP2-74462G
DyLight 550
NBP2-74462R
DyLight 594
NBP2-74462DL594
DyLight 650
NBP2-74462C
DyLight 680
NBP2-74462FR
DyLight 755
NBP2-74462IR
FITC
NBP2-74462F
HRP
NBP2-74462H
Janelia Fluor 525
NBP2-74462JF525
Janelia Fluor 549
NBP2-74462JF549
Janelia Fluor 646
NBP2-74462JF646
Janelia Fluor 669
NBP2-74462JF669
PE
NBP2-74462PE
PE/Atto594
NBP2-74462PEATT594
PE/Cy5.5
NBP2-74462PECY55
PE/Cy7
NBP2-74462PECY7
PerCP
NBP2-74462PCP
Unconjugated
NBP2-00597
mFluor Violet 450 SE
NBP2-74462MFV450
mFluor Violet 500 SE
NBP2-74462MFV500
mFluor Violet 610 SE
NBP2-74462MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00597
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TBC1D4 Antibody (OTI5E6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human TBC1D4 (NP_0055647) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TBC1D4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
146.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free

  • Akt substrate of 160 kDa
  • AS160Acrg embryonic lethality minimal region ortholog
  • DKFZp779C0666
  • KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
  • TBC1 domain family member 4
  • TBC1 domain family, member 4

Background

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Glut4 Antibody -
BSA Free
NBP1-49533
Flow (Intracellular) Glut4 Antibody - BSA Free
Western Blot Glut4 Antibody - BSA Free
Western Blot Glut4 Antibody - BSA Free
(4 Reviews)
(41 Publications)
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AMPK alpha 2
Antibody [Unconjugate
AF2850
Western Blot AMPK alpha 2 Antibody [Unconjugated]
Simple Western AMPK alpha 2 Antibody [Unconjugated]
Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
AMPK alpha 1
Antibody (2B7) - BSA
NBP2-22127
Flow Cytometry AMPK alpha 1 Antibody (2B7) - BSA Free
Flow Cytometry AMPK alpha 1 Antibody (2B7) - BSA Free
ELISA AMPK alpha 1 Antibody (2B7) - BSA Free
(12 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
AMPK beta 1
Antibody [Unconjugate
AF2854
Western Blot AMPK beta 1 Antibody [Unconjugated]
Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]
Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
PYK2/FAK2 [p
Tyr402] Antibody (592 ...
MAB6210
Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]
Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: Simple Western, WB
TBC1D1 Antibody
NBP1-54654
Western Blot TBC1D1 Antibody
Western Blot TBC1D1 Antibody
Immunohistochemistry-Paraffin TBC1D1 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
IRS1 Antibody
NB100-82001
Immunohistochemistry-Paraffin IRS1 Antibody
Western Blot IRS1 Antibody
Immunocytochemistry/ Immunofluorescence IRS1 Antibody
(4 Publications)
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Leucyl-cystinyl
Aminopeptidase/LNPEP ...
AF6386
Western Blot Leucyl-cystinyl Aminopeptidase/LNPEP Antibody
Species: Hu
Applications: IP, WB
Insulin Receptor
beta Antibody
NBP2-12793
Immunohistochemistry-Paraffin Insulin Receptor beta Antibody
Immunoprecipitation Insulin Receptor beta Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody
Western Blot TOR/mTOR [p Ser2448] Antibody
Data TOR/mTOR [p Ser2448] Antibody
(2 Reviews)
(9 Publications)
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
ATIC Antibody
NBP1-89192
Western Blot ATIC Antibody
Western Blot ATIC Antibody
Immunohistochemistry-Paraffin ATIC Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
14-3-3 Antibody
NBP2-27202
Simple Western 14-3-3 Antibody
Immunocytochemistry/ Immunofluorescence 14-3-3 Antibody
Western Blot 14-3-3 Antibody
(1 Review)
Species: Ca, Ch, ChHa, Fe, Hu, Mu, Ma-Op, Po, Pm, Rt
Applications: ICC/IF, Simple Western, WB
Glut1 Antibody -
BSA Free
NB110-39113
Western Blot Glut1 Antibody - BSA Free
Flow Cytometry Glut1 Antibody - BSA Free
Western Blot Glut1 Antibody - BSA Free
(7 Reviews)
(62 Publications)
Species: Hu, Mu, Rb, Rt
Applications: ChIP, ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB
RPL6 Antibody
NBP2-20216
Immunohistochemistry-Paraffin RPL6 Antibody
Immunohistochemistry-Paraffin RPL6 Antibody
Immunohistochemistry-Paraffin RPL6 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
MED10 Antibody
NBP1-81328
Western Blot MED10 Antibody
Immunocytochemistry/ Immunofluorescence MED10 Antibody
Species: Hu
Applications: ICC/IF, WB
TM4SF1/L6 Antibody
(877621) [Unconjugate ...
MAB8164
Immunocytochemistry TM4SF1/L6 Antibody (877621) [Unconjugated]
Flow Cytometry TM4SF1/L6 Antibody (877621) [Unconjugated]
Flow Cytometry TM4SF1/L6 Antibody (877621) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC

Publications for TBC1D4 Antibody (NBP2-74462) (0)

There are no publications for TBC1D4 Antibody (NBP2-74462).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TBC1D4 Antibody (NBP2-74462) (0)

There are no reviews for TBC1D4 Antibody (NBP2-74462). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBC1D4 Antibody (NBP2-74462) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TBC1D4 Products

Research Areas for TBC1D4 Antibody (NBP2-74462)

Find related products by research area.

Blogs on TBC1D4

There are no specific blogs for TBC1D4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TBC1D4 Antibody (OTI5E6) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol TBC1D4