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T-bet/TBX21 Antibody (525831) [Unconjugated]

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Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and 10 ng/mL Recombinant Human IL‑12 (Catalog #219-IL) for 5 days to induce Th1 cell development were stained with Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB53851) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and 10 ng/mL Recombinant Human IL‑12 (Catalog #219-IL) for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
525831
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB53851U-100UG
Alexa Fluor 405
FAB53851V-100UG
Alexa Fluor 488
FAB53851G-100UG
Alexa Fluor 488
IC53851G-025
Alexa Fluor 532
FAB53851X
Alexa Fluor 594
FAB53851T-100UG
Alexa Fluor 647
FAB53851R-100UG
Alexa Fluor 700
FAB53851N-100UG
Alexa Fluor 750
FAB53851S-100UG
Allophycocyanin
FAB53851A
Biotin
FAB53851B
CoraFluor 1
FAB53851CL1
DyLight 350
FAB53851D
DyLight 405
FAB53851E
DyLight 488
FAB53851K
DyLight 550
FAB53851L
DyLight 594
FAB53851M
DyLight 650
FAB53851W
DyLight 680
FAB53851Y
DyLight 755
FAB53851Z
FITC
FAB53851F
HRP
FAB53851H
Janelia Fluor 525
FAB53851JF525
Janelia Fluor 549
FAB53851I
Janelia Fluor 646
FAB53851J
Janelia Fluor 669
FAB53851JF669
PE
FAB53851P
PerCP
FAB53851C
mFluor Violet 450 SE
FAB53851MFV450
mFluor Violet 500 SE
FAB53851MFV500
mFluor Violet 610 SE
FAB53851MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB53851-SP
Carrier Free
IC53851G-025
Carrier Free
FAB53851V-100UG
Carrier Free
FAB53851U-100UG
Carrier Free
FAB53851T-100UG
Carrier Free
FAB53851S-100UG
Carrier Free
FAB53851R-100UG
Carrier Free
FAB53851N-100UG
Carrier Free
FAB53851G-100UG
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T-bet/TBX21 Antibody (525831) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human T‑bet/TBX21
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T‑bet/TBX21 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
MAB53851 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for T-bet/TBX21 Antibody (525831) [Unconjugated]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma , and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for T-bet/TBX21 Antibody (MAB53851) (0)

There are no publications for T-bet/TBX21 Antibody (MAB53851).
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Review for T-bet/TBX21 Antibody (MAB53851) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB53851:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen T-bet/TBX21 MAB53851
Enlarge 		5
reviewed by:
Verified Customer
 IHC-Fr Human 03/30/2020
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTonsil
SpeciesHuman
LotN/A

FAQs for T-bet/TBX21 Antibody (MAB53851). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunohistochemistry-Frozen T-bet/TBX21 MAB53851
Enlarge
 Verified Customer
03/30/2020
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol TBX21
Uniprot