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T-bet/TBX21 Antibody (4B10) [PerCP/Cy5.5]

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Flow Cytometry: T-bet/TBX21 Antibody (4B10) [PerCP/Cy5.5] [NBP1-42835] - Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of Anti-Human/Mouse T-bet PerCP-Cy5.5 using the Foxp3 Fixation/Permeabilization Buffers . Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: T-bet/TBX21 Antibody (4B10) [PerCP/Cy5.5] [NBP1-42835] - Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of Anti-Human/Mouse T-bet PerCP-Cy5.5 using ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications Flow
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP/Cy5.5
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-43298AF350
Alexa Fluor 405
NBP1-43298AF405
Alexa Fluor 488
NBP1-43298AF488
Alexa Fluor 532
NBP1-43298AF532
Alexa Fluor 594
NBP1-43298AF594
Alexa Fluor 647
NBP1-43298AF647
Alexa Fluor 700
NBP1-43298AF700
Alexa Fluor 750
NBP1-43298AF750
Allophycocyanin
NBP1-43298APC
Allophycocyanin/Cy7
NBP1-43298APCCY7
Biotin
NBP1-43298B
CoraFluor 1
NBP1-43298CL1
DyLight 350
NBP1-43298UV
DyLight 405
NBP1-43298V
DyLight 488
NBP1-43298G
DyLight 550
NBP1-43298R
DyLight 594
NBP1-43298DL594
DyLight 650
NBP1-43298C
DyLight 680
NBP1-43298FR
DyLight 755
NBP1-43298IR
FITC
NBP1-43298F
HRP
NBP1-43298H
Janelia Fluor 525
NBP1-43298JF525
Janelia Fluor 549
NBP1-43298JF549
Janelia Fluor 646
NBP1-43298JF646
Janelia Fluor 669
NBP1-43298JF669
PE
NBP1-43298PE
PE/Atto594
NBP1-43298PEATT594
PE/Cy5.5
NBP1-43298PECY55
PE/Cy7
NBP1-43298PECY7
PerCP
NBP1-43298PCP
PerCP/Cy5.5
NBP1-42835-0.025mg
Unconjugated
NBP1-43298-0.1mg
mFluor Violet 450 SE
NBP1-43298MFV450
mFluor Violet 500 SE
NBP1-43298MFV500
mFluor Violet 610 SE
NBP1-43298MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-43298-0.1mg
BSA Free
NBP1-43298-0.1mg
BSA Free
NBP1-43298-0.025mg
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T-bet/TBX21 Antibody (4B10) [PerCP/Cy5.5] Summary

Immunogen
The immunogen for this antibody was TBet.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This 4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for T-bet/TBX21 Antibody (4B10) [PerCP/Cy5.5]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

The 4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for T-bet/TBX21 Antibody (NBP1-42835). (Showing 1 - 1 of 1 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TBX21