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Syndecan-1/CD138 Antibody (300506) [Fluorescein]

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T1165 mouse plasmacytoma cell line treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) was stained with Rat Anti-Mouse Syndecan-1/CD138 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2966F, filled ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
300506
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

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Syndecan-1/CD138 Antibody (300506) [Fluorescein] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Syndecan‑1/CD138 isoform 1
Gln18-Glu252
Accession # P18828
Specificity
Detects mouse Syndecan‑1/CD138 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Syndecan-1, recombinant mouse (rm) Syndecan-3 or rmSyndecan-4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
SDC1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publication using
FAB2966F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-1/CD138 Antibody (300506) [Fluorescein]

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the Syndecan family of Type 1 transmembrane proteins (1, 2). The four Syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 22 aa signal sequence, a 233 aa extracellular domain (ECD) that includes three closely spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 21 aa TM segment, and a 35 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site (4). The ECD is variably modified by GAGs, producing molecular weights of 120‑200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Mouse Syndecan-1 ECD shares 70% and 87% aa identity with the ECD of human and rat Syndecan-1, respectively. Alternative splicing in mouse generates an isoform with an internal deletion of 44 aa from the ECD (5). Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (6, 7). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (6‑10). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (7, 9, 11). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (12‑14). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (14, 15). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (6).
  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
  3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  4. Saunders, S. et al. (1989) J. Cell Biol. 108:1547.
  5. Romaris, M. et al. (1999) J. Biol. Chem. 274:18667.
  6. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
  7. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
  8. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
  9. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
  10. Vanhoutte, D. et al. (2007) Circulation 115:475.
  11. Li, Q. et al. (2002) Cell 111:635.
  12. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
  13. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
  14. Derksen, P.W.B. et al. (2002) Blood 99:1405.
  15. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Syndecan-1/CD138 Antibody (FAB2966F)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol SDC1
Uniprot