![Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Western-Blot-NBP2-37282-img0004.jpg "Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 1A3H4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Syndecan-1/CD138 (AA: 28-171) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SDC1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Theoretical MW | 32.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP2-37282 | Applications | Species |
|---|---|---|
| Ponandai-Srinivasan S, Saare M, et al. Syndecan-1 modulates the invasive potential of endometrioma via TGF-beta signalling in a subgroup of women with endometriosis. Hum Reprod 2020-10-01 [PMID: 32897364] (Simple Western, Human) | Simple Western | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SDC1 |
![Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Immunocytochemistry-Immunofluorescence-NBP2-37282-img0005.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of rectum cancer tissues using SDC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Immunohistochemistry-NBP2-37282-img0008.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of rectum cancer tissues using SDC1 mouse mAb with DAB staining.")
![Flow Cytometry: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Flow-Cytometry-NBP2-37282-img0006.jpg "Flow Cytometry: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of HepG2 cells using SDC1 mouse mAb (green) and negative control (red).")
![Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Western-Blot-NBP2-37282-img0002.jpg "Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)")
![Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Western-Blot-NBP2-37282-img0003.jpg "Western Blot: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of ovarian cancer tissues using SDC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-Immunohistochemistry-NBP2-37282-img0007.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Analysis of ovarian cancer tissues using SDC1 mouse mAb with DAB staining.")
![ELISA: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-1A3H4-ELISA-NBP2-37282-img0001.jpg "ELISA: Syndecan-1/CD138 Antibody (1A3H4) [NBP2-37282] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")