Novus Biologicals products are now on bio-techne.com

SV2A Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Analysis in human cerebral cortex and small intestine tissues using HPA007863 antibody. Corresponding SV2A RNA-seq dat
Immunocytochemistry/ Immunofluorescence: SV2A Antibody [NBP1-82964] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human small intestine shows no positivity in glandular cells.
Western Blot: SV2A Antibody [NBP1-82964] - Analysis of SV2A in (1) undifferentiated SH-SY5Y, (2) differentiated SH-SY5Y (neurons) and (3) mouse brain using anti-SV2A antibody. 30ug of protein/lane. Image from verified ...read more
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: SV2A Antibody [NBP1-82964] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunoprecipitation: SV2A Antibody [NBP1-82964] - Staining of neuroblastoma cells SH-SY5Y differentiated into neurons. Lane (1) 10ug of input, 500ug used for IP. 10ul of antibody used for incubation with lysate ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

SV2A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SDGYYRGEGTQDEEEGGASSDATEGHDEDDEIYEGEYQGIPRAESGGKGERMADGAPLAGVRGGLSDGEGPPGGRGEAQRRKEREELAQQYEAILRECGHGRFQW
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SV2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation Reported in scientific literature (PMID: 24284412)/verified customer review.
  • Western Blot Reported in scientific literature (PMID: 24284412)/verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
Control Peptide
SV2A Protein (NBP1-82964PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-82964 in the following applications:

Publications
Read Publications using
NBP1-82964 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SV2A Antibody

  • KIAA0736
  • KIAA0736SV2
  • SV2
  • SV2A
  • synaptic vesicle glycoprotein 2A

Background

SV2A (Synaptic Vesicle protein 2A) is an integral membrane glycoproteins present in synaptic vesicles. SV2s have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-653
Species: Ch, Fe, Hu, Pm, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-00795
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-92462
Species: Hu
Applications: IHC, IHC-P
MAB4364
Species: Rt
Applications: ICC, IHC, IP, KO, WB
NBP1-86239
Species: Hu
Applications: IHC, IHC-P
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
NBP2-45499
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP3-13165
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB100-91348
Species: Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-46641
Species: Hu, Mu, Rt, RM
Applications: IHC, IHC-Fr, IHC-P
NB300-104
Species: Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NB300-595
Species: Bv, Mu, Pm, Rt
Applications: Flow, IF, IHC, IHC-P, IP, WB
NB110-41404
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP2-46349
Species: Hu
Applications: IHC, IHC-P, WB
NB120-15160
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-88191
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-82964
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP

Publications for SV2A Antibody (NBP1-82964)(3)

Reviews for SV2A Antibody (NBP1-82964) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Mouse.

Reviews using NBP1-82964:
Filter by Applications
WB
(1)
IP
(1)
All Applications
Filter by Species
Human
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SV2A NBP1-82964
Enlarge
4
reviewed by:
Jennifer Fox
WB Human and Mouse 01/28/2015
View

Summary

ApplicationWestern Blot
Sample TestedL1: undifferentiated SH-SY5Y, L2: differentiated SH-Sy5Y (neurons), L3: Mouse Brain
SpeciesHuman and Mouse
LotA74250
Immunoprecipitation SV2A NBP1-82964
Enlarge
4
reviewed by:
Verified Customer
IP Human 01/28/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedNeuroblastoma cells SH-SY5Y differentiated into neurons
SpeciesHuman
LotA74250

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SV2A Antibody (NBP1-82964) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Jennifer Fox
01/28/2015
Application: WB
Species: Human and Mouse

Verified Customer
01/28/2015
Application: IP
Species: Human

Bioinformatics

Gene Symbol SV2A