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Rab3A Antibody (OTI1G3)

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Western Blot: Rab3A Antibody (OTI1G3) [NBP2-45499] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: Rab3A Antibody (OTI1G3) [NBP2-45499] - Staining of paraffin-embedded Human lymphoma tissue using anti-RAB3A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Rab3A Antibody (OTI1G3) [NBP2-45499] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RAB3A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Rab3A Antibody (OTI1G3) [NBP2-45499] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RAB3A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Rab3A Antibody (OTI1G3) [NBP2-45499] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RAB3A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Rab3A Antibody (OTI1G3) [NBP2-45499] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RAB3A mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Rab3A Antibody (OTI1G3) Summary

Immunogen
Full length human recombinant protein of human RAB3A(NP_002857) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAB3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rab3A Antibody (OTI1G3)

  • RAB3A, member RAS oncogene family
  • RAS-associated protein RAB3A
  • ras-related protein Rab-3A

Background

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3's are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAB3A