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STEAP1 Antibody (1057323) [Unconjugated]

Images

 
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) followed by HRP-conjugated Anti-Mouse IgG ...read more
HEK293 transfected with Human STEAP1 and eGFP were stained with eGFP and either (A) Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by ...read more
LnCaP cells were stained with Mouse Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB5587, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
1057323
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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STEAP1 Antibody (1057323) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human STEAP-1
Met1-Trp71
Accession # Q9UHE8
Specificity
Detects human STEAP-1 in direct ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STEAP1 Antibody (1057323) [Unconjugated]

  • EC 1.16.1.-
  • PRSS24
  • PRSS24MGC19484
  • six transmembrane epithelial antigen of the prostate 1
  • six transmembrane epithelial antigen of the prostate
  • Six-transmembrane epithelial antigen of prostate 1
  • STEAP1
  • STEAPmetalloreductase STEAP1

Background

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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