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SS18L1 Antibody

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Western Blot: SS18L1 Antibody [NBP2-20485] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Western Blot: SS18L1 Antibody [NBP2-20485] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SS18L1 Antibody [NBP2-20485] - SS18L1 antibody detects CREST protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 ...read more
Western Blot: SS18L1 Antibody [NBP2-20485] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: SS18L1 Antibody [NBP2-20485] - Rat tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Western Blot: SS18L1 Antibody [NBP2-20485] - U87-MG whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [C1C3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SS18L1 Antibody [NBP2-20485] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREST protein stained by CREST antibody [C1C3 diluted at 1:500. Blue: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SS18L1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SS18L1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SS18L1
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SS18L1 Antibody

  • calcium-responsive transactivator
  • CRESTMGC78386
  • KIAA0693MGC26711
  • LP2261
  • SS18-like protein 1
  • synovial sarcoma translocation gene on chromosome 18-like 1
  • SYT homolog 1

Background

Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SS18L1
Uniprot