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Src Antibody [Unconjugated]

 (7 Publications)
 
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Src at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL‑60 human acute promyelocytic ...read more
Src was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) overnight at 4 °C. Tissue was ...read more
Src was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) at 15 µg/mL overnight at 4 °C. ...read more
Src was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) at 10 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Src at approximately 62 ...read more
Lasp1 affects tissue architecture in the synovium of hTNFtg mice.a H&E stainings (scale bar: 50 µm) of paraffin sections from hind paws from 12 weeks old wt, Lasp1−/−, hTNFtg and hTNFtg/Lasp1−/− female mice ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3389-SP
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Src Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Src
Met1-Ala79
Accession # P12931
Specificity
Detects endogenous human, mouse, and rat Src in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SRC
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF3389 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Src Antibody [Unconjugated]

  • ASV
  • c-Src
  • EC 2.7.10
  • EC 2.7.10.2
  • pp60c-src
  • Rous sarcoma
  • RSVgp4
  • Src
  • tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

The Src family of proteins are intracellular tyrosine kinases involved in cell proliferation, differentiation, motility, and survival. Src family activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Autophosphorylation in the activation loop of the kinase domain (Y419 of human Src) up‑regulates the enzyme, while phosphorylation in the C-terminal tail (Y530 of human Src) down‑regulates activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Src Antibody [Unconjugated]
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Publications for Src Antibody (AF3389)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(3)
Western Blot
(2)
All Applications
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Human
(2)
Mouse
(3)
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Showing Publications 1 - 7 of 7.
Publications using AF3389 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Priscilla Y. Hwang, Jairaj Mathur, Yanyang Cao, Jose Almeida, Jiayu Ye, Vasilios Morikis et al. A Cdh3-beta -catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration Developmental Cell 2023-01-09 [PMID: 36626870]
L Nie, K Sun, Z Gong, H Li, JP Quinn, M Wang Src Family Kinases Facilitate the Crosstalk between CGRP and Cytokines in Sensitizing Trigeminal Ganglion via Transmitting CGRP Receptor/PKA Pathway Cells, 2022-11-04;11(21):. 2022-11-04 [PMID: 36359895] (IHC, Mouse) IHC Mouse
L Nie, L Jiang, JP Quinn, BD Grubb, M Wang TRPA1-Mediated Src Family Kinases Activity Facilitates Cortical Spreading Depression Susceptibility and Trigeminovascular System Sensitization International Journal of Molecular Sciences, 2021-11-12;22(22):. 2021-11-12 [PMID: 34830154] (IHC, Mouse) IHC Mouse
Rubén A. Bartolomé, Javier Robles, Ángela Martin‐Regalado, Laura Pintado‐Berninches, Miranda Burdiel, Marta Jaén et al. CDH6‐activated alpha IIb beta 3 crosstalks with alpha 2 beta 1 to trigger cellular adhesion and invasion in metastatic ovarian and renal cancers Molecular Oncology 2021-07-01 [PMID: 33715292]
RA Bartolomé, M Jaén, JI Casal An IL13R?2 peptide exhibits therapeutic activity against metastatic colorectal cancer Br. J. Cancer, 2018-10-15;0(0):. 2018-10-15 [PMID: 30318506] (Western Blot, Human) Western Blot Human
JH Pak, Q Bashir, IK Kim, SJ Hong, S Maeng, YY Bahk, TS Kim Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin ?4-FAK/Src signaling pathway Mol. Biochem. Parasitol, 2017-03-08;0(0):. 2017-03-08 [PMID: 28286026] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRC
Uniprot