![Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0013.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.31 mg/ml |
| Immunogen | Full length human recombinant protein of human SPR (NP_003115) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 27.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.31 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for SPR Antibody (NBP2-03256)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunocytochemistry-Immunofluorescence-NBP2-03256-img0012.jpg "Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0011.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.")
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0002.jpg "Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.")
![Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0003.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0004.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0005.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0006.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0007.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0008.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0009.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0010.jpg "Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.")
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0001.jpg "Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]](https://images.novusbio.com/images2/TFF2_MAB4077_Immunohistochemistry_21485.jpg)
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")