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SPAG9 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

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SPAG9 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human SPAG9.

Source: E. coli

Amino Acid Sequence: NLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVL

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
SPAG9
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56914.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking tide related information and a protocol, click here.

Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for SPAG9 Recombinant Protein Antigen

  • Cancer/testis antigen 89
  • c-Jun NH2-terminal kinase-associated leucine zipper protein
  • C-Jun-amino-terminal kinase-interacting protein 4
  • CT89JIP4
  • FLJ14006
  • FLJ26141
  • FLJ34602
  • HLC4
  • HLC-6
  • HSS
  • Human lung cancer oncogene 6 protein
  • JLPPHETSYD1FLJ13450
  • JNK interacting protein
  • JNK/SAPK-associated protein
  • JNK-associated leucine-zipper protein
  • JNK-interacting protein 4
  • KIAA0516JIP-4
  • lung cancer oncogene 4
  • MAPK8IP4
  • Max-binding protein
  • MGC117291
  • MGC14967
  • MGC74461
  • Mitogen-activated protein kinase 8-interacting protein 4
  • PIG6
  • proliferation-inducing gene 6
  • Proliferation-inducing protein 6
  • Protein highly expressed in testis
  • sperm associated antigen 9
  • Sperm surface protein
  • Sperm-associated antigen 9
  • Sperm-specific protein
  • Sunday driver 1

Background

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SPAG9