SOD1/Cu-Zn SOD Antibody

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 1 application: WB.

Showing Publications 1 - 2 of 2.

Publications using NBP1-31204	Applications	Species
Dickson MJ, Kvidera SK, Horst EA et al. Impacts of chronic and increasing lipopolysaccharide exposure on production and reproductive parameters in lactating Holstein dairy cows J. Dairy Sci. 2019-02-06 [PMID: 30738665] (WB, Bovine)	WB	Bovine
Gasperini L, Rossi A, Cornella N et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol. Biol. Cell. 2018-10-24 [PMID: 30354839] (WB, Human)	WB	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Pig	11/17/2016	View Summary Application Western Blot Sample Tested Ovary tissue Species Pig Comments Comments Sample Information:      Treatment: None. Run on control tissue Controls:      Positive Control: None      Negative Control: Primary only, secondary only, and secondary with IgG all negative      Loading Control: Ponceau S stain Total Protein Loaded: 40 ug/well Electrophoresis: Gel Percentage: Gradient, 4-20% Electrophoresis Conditions:      Voltage: 50V then 90V      Time: 5 min then 1 hr Membrane Transfer:      Method (Submersion/Semi-dry): Semi-dry using iBlot2 system      Membrane Type (PVDF/Nitrocellulose): Nitrocellulose      Time: 7 min (iBlot2 Protocol 0) Blocking:      Blocking Solution: 5% BSA in .2% PBST      Time: 1.5 hours Primary Antibody:      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST           Incubation Time: overnight           Incubation Temperature: 4°C Washing Conditions:      Wash Solution: .2% PBST      Time and Repetitions: 3X, 10 minutes each Secondary Antibody Manufacturer and Catalog #: Cell Signaling, #7074 Secondary description: goat anti-rabbit      Dilution: 1:1000      Diluent Buffer: 5% BSA in .2% PBST      Incubation Time: 1 hour      Incubation Temperature: room temperature Detection Method: Detection: SignalFire ECL reagent (Cell Signaling) Procedure: ECL on blot 3 min Development Time: 5 min Molecular weight of band(s): 13 kDa

Array NBP1-31204

• SOD1/Cu-Zn SOD Antibodies
• SOD1/Cu-Zn SOD Antibody Pair
• SOD1/Cu-Zn SOD ELISA Kits
• SOD1/Cu-Zn SOD Lysate
• SOD1/Cu-Zn SOD Proteins